摘要
通过对３种研磨提取液、５种凝胶及电泳缓冲系统以及１４种等位酶系统的筛选。结果表明、红皮云 杉同工酶种子研磨提取液采用ｃ、凝胶及电泳缓冲系统采用８、#１１较好。#８胶可染ＡＡＴ、ＡＤＨ、ＧＤＨ、 ＤＩＡ．#１１胶可染ＭＤＨ、ＭＥ、ＩＤＨ、６ＰＧＤ、ＰＧＩ、ＡＣＯ，１６个位点可作为红皮云杉遗传标记。
The seeds of PI'cea koraiensis from 11 sites throughout the northeast area in China were tested with 3 kinds of enzyme extraction bufTers. 5 kinds of gel electrophoresis buffers and 14 kinds of enzyme system. The results indicated that c extraction buffer and #8. #l I gel electrophoresis buffer systems were used better in the seeds of P. koraiensis than others. #8 gel electrophoresis was used to stain well in AAT, GDH and DIA. while #if in MDH, ME, IDH. 6PGD* PGI and ACO. 16 allozyme loci are taken as genetic marker for P. koraiensis.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2000年第2期9-12,共4页
Journal of Northeast Forestry University
基金
黑龙江省重点课题及国家"九五"科技攻关项目
