摘要
目的探讨重组人脑利钠肽（rhBNP）后适应对兔急性缺血／再灌注（I／R）心肌的保护作用及其机制。方法将36只健康日本大耳白兔按数字表法随机分为三组（每组12只），即假手术组、I／R组（缺血40rain后再灌注180min）及BNP＋I／R组（I／R前5min以0．01μg／kg静脉给予rhBNP）。假手术组开胸于左冠回旋支只穿线但不结扎，观察180min结束；I／R组及BNP＋I／R组：缺血40min，分别再灌注180min后处死。进行心肌磷酸肌酸激同工酶（CK．MB）含量检测，观察心肌HE染色后病理形态变化及BCI-2、Bax表达的检测。结果与假手术组相比，I／R组和BNP＋I／R组CK—MB水平均显著增高（t=3．047、3．032，均P〈0．01）；与I／R组相比，BNP＋I／R组心肌酶明显升高（t=3．067，P〈0．01）。与假手术组相比，I／R组及BNP＋L／R组均可见到凋亡细胞，但BNP＋IYR组凋亡细胞明显减少，UR组Bax的表达增加，Bcl-2的表达降低。与I／R组相比，BNP＋I／R组Bax的表达明显升高，而Bcl-2的表达明显降低。结论rhBNP可减少梗死后心肌的损伤；可以增加梗死后心肌细胞抑制凋亡基因（Bcl-2）的表达，从而减少心肌细胞的凋亡。
Objective To study the protective effect of recombinant human brain natriuretic peptide （rhBNP） postconditioning on acute myocardial ischemia-reperfnsion （I/R） injury in rabbits and its mechanism. Methods Thirty-six healthy rabbits were randomly divided into three groups： sham-operated group, I/R group and rhBNP postconditioning group, 12 rabbits in each group. Blood vessels were not occluded in sham-operated group, a 40-minute occlusion of the left coronary vessel was followed by a 180-minute reperfusion of physiological saline in I/ R group and rhBNP was injected 5 minutes before and throughout the whole reperfusion in rhBNP postconditioning group. Serum CK-MB was detected and recorded. Expressions of Bcl-2 and Bas were examined. Results The level of CK-MB markedly increased in I/R group compared with that in sham-operated group （ P 〈 0.01 ）. The elevation of CK-MB obviously reduced in rhBNP postconditioning group. Myocardial cell apoptosis significantly reduced in rhBNP postconditioning group compared with that in I/R group. The expression of Bax increased and the expression of Bcl-2 decreased in I/R group, which were contrary to those in rhBNP postconditioning group. Conclusion Biochemical ex- aminations show that rhBNP reduces myocardial infarction injury and increases the expression of Bcl-2, thus lowering myocardial apoptosis.
出处
《中国基层医药》
CAS
2013年第23期3526-3528,共3页
Chinese Journal of Primary Medicine and Pharmacy
基金
基金项目：黑龙江省科技厅青年基金资助项目（QC2011c123）
关键词
心肌梗死
缺血预处理
心肌
利钠肽
脑
兔
Myocardial infarction
Ischemic preconditioning, myocardial
Natriuretic peptide, brain
Rabbits
