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上调miR-187*表达对人结肠癌细胞株增殖活性的影响 被引量:8

Up-regulation of microRNA-187* inhibits proliferation of human colon cancer cell line HCT116
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摘要 目的:研究微小RNA-187*(miR-187*)在人结肠癌细胞株及正常结肠组织中的表达,同时分析miRNA-187*上调对人结肠癌细胞增殖和细胞周期的影响。方法:选取3例结直肠癌组织及配对正常黏膜组织进行miRNA芯片检测,筛选出大肠癌组织中异常表达的miRNA;提取8株结肠癌细胞株和10例正常结肠组织中总RNA,采用Taqman实时定量PCR方法检测miR-187*的表达;预测miR-187*的靶基因,采用实时定量PCR方法检测靶基因的表达;采用脂质体介导的转染方法将miR-187*模拟物转染人结肠癌细胞株HCT116;检测转染后miR-187*和靶基因的表达;通过MTS试剂盒检测细胞增殖能力,流式细胞术检测miR-187*对细胞周期的影响。结果:miRNA芯片检测结直肠癌组织中miR-187*的表达较正常黏膜明显降低;miR-187*在结肠癌细胞株中的表达较正常结直肠黏膜组织明显下调;而B细胞特异性莫洛尼小鼠白血病病毒整合位点1(BMI-1)mRNA在结肠癌细胞株中表达较正常黏膜组织中明显增高;转染miR-187*模拟物后,miR-187*表达明显增加;同时miR-187*的高表达可以显著抑制BMI-1 mRNA的水平;miR-187*模拟物转染组和阴性对照组相比,细胞增殖活力明显受抑制(P<0.05),同时增殖细胞核抗原表达亦明显降低;细胞周期检测结果显示,miR-187*模拟物转染组G2/M期细胞增多,和阴性对照组间差异有统计学意义。结论:miR-187*在人结肠癌细胞株中表达下调;上调miR-187*表达可抑制结肠癌细胞增殖活性,影响结肠癌细胞周期;miR-187*可能通过抑制BMI-1在结肠癌中发挥抑癌作用。 AIM: To investigate the expression of microRNA-187 * (miR-187 * ) in human colon cancer cell lines and normal colon tissues, and to determine the effects of miR-187 * up-regulation on the proliferation and cell cycle of human colon cancer cell line HCT116. METHODS: The expression profiling of miRNAs in 3 colorectal adenocarcinoma samples and their matched normal tissue samples was performed using miRNA microarray chip. Total RNA was isolated from 8 colon cancer cell lines and 10 normal colon tissues. The miR-187 * level was detected by Taqman real-time RT-PCR. B-cell-specific Moloney murine leukemia virus integration site 1 ( BMI-1 ), the possible target of miR-187 * , was also detected. Synthetic miR-187 * mimics were transfected into HCT116 cell line by LipofectamineTM 2000. The mRNA expression of miR-187 * and BMI-1 in HCT116 cell line was measured by real-time RT-PCR. Cell growth and cell cycle were assayed by MTS method and flow cytometry. RESULTS: miR-187 * was found to be differentially expressed between colorectal adenocarcinoma and normal tissues. The expression of miR-187 * in 8 colon cancer cell lines was down-regulated, while BMI-1 mRNA was up-regulated. Compared with blank control group, miR-187 * expression was remarkably increased after transfection with miR-187 * mimics, and ectopic expression of miR-187 * significantly inhibited the mRNA expression of BMI-1. The cell growth was inhibited in miR-187 * mimics group, and proliferating cell nuclear antigen(PCNA) mRNA expression was decreased. The cells at G2/M phase in miR-187 * mimics group were significantly increased. CONCLUSION: miR-187 * is down-regulated in human colon cancer cell lines. Up-regulation of miR-187 * not only inhibits the proliferation but also influence the cell cycle of HCT116 cells, which might act as a tumor suppressor in colorectal cancer by inhibiting the expression of BMI-1.
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2013年第11期1946-1951,共6页 Chinese Journal of Pathophysiology
基金 国家自然科学基金资助项目(No.81072034) 河北省自然科学研究计划(No.C2011206103) 河北省国际合作项目(No.12396105D)
关键词 结肠肿瘤 微小RNA-187 细胞增殖 细胞周期 Colon neoplasms MicroRNA-187 * Cell proliferation Cell cycle
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参考文献18

  • 1Ambros V. The function of animal microRNAs[J].Nature,2004,(7006):350-355.doi:10.1038/nature02871. 被引量:1
  • 2Rosenfeld N,Aharonov R,Meiri E. MicroRNAs accurately identify cancer tissue origin[J].Nat Biotech-nol,2008,(04):462-469. 被引量:1
  • 3Lim LP,Lau NC,Garrett-Engele P. Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs[J].Nature,2005,(7027):769-773. 被引量:1
  • 4Zhang N,Li X,Wu CW. microRNA-7 is a novel inhibitor of YY1 contributing to colorectal tumorigenesis[J].Oncogene,2013,(42):5078-5088. 被引量:1
  • 5He X,Dong Y,Wu CW. MicroRNA-218 inhibits cell cycle progression and promotes apoptosis in colon cancer by downregulating BMI1 polycomb ring finger oncogene[J].Molecular Medicine,2012.1491-1498. 被引量:1
  • 6Chen X,Guo X,Zhang H. Role of miR-143 targeting KRAS in colorectal tumorigenesis[J].Oncogene,2009,(10):1385-1392.doi:10.1038/onc.2008.474. 被引量:1
  • 7Liu L,Chen L,Xu Y. microRNA-195 promotes apoptosis and suppresses tumorigenicity of human colorectal cancer cells[J].Biochemical and Biophysical Research Communications,2010,(02):236-240. 被引量:1
  • 8Liang L,Li X,Zhang X. MicroRNA-137,an HMGA1 target,suppresses colorectal cancer cell invasion and metastasis in mice by directly targeting FMNL2[J].Gastroenterology,2013,(03):624-635.e4. 被引量:1
  • 9Ferracin M,Pedriali M,Veronese A. microRNA profiling for the identification of cancers with unknown primary tissue-of-origin[J].Journal of Pathology,2011,(01):43-53. 被引量:1
  • 10Chao A,Lin CY,Lee YS. Regulation of ovarian cancer progression by microRNA-187 through targeting Disabled homolog-2[J].Oncogene,2012,(06):764-775. 被引量:1

二级参考文献42

  • 1张军,张旭,许凯,傅斌,郎斌,胡建庭,艾星,史涛坪,朱宏刚,陈军.膀胱移行细胞癌患者血浆p14基因异常甲基化检测的意义[J].中华实验外科杂志,2007,24(3):333-335. 被引量:6
  • 2van Lohuizen M, Verbeek S, Scheijen B, et al. Identification of cooperating oncogenes in Ep,- myc transgenic mice by provirus tagging[ J]. Cell, 1991,65 (5) :737 - 752. 被引量:1
  • 3Reinisch C, Kandutsch S, Uthman A, et al. BMI - 1 : a protein expressed in stem cells, specialized ceils and tumors of the gastrointestinal tract [ J ]. Histol Histopathol, 2006,21 (11) :1143 - 1149. 被引量:1
  • 4Park IK,Morrison SJ, Clarke MF. Bmil, stem cells, and senescence regulation [ J ]. J Clin Invest, 2004,113 ( 2 ) : 175 - 179. 被引量:1
  • 5Liu Y, Yang Y, Xu H, et al. Implication of USP22 in the regulation of BMI - 1, c - Myc, p16^INK4a, p14ARF, and cyclin D2 expression in primary eoloreetal carcinomas [ J ]. Diagu Mol Pathol,2010,19 (4) : 194 - 200. 被引量:1
  • 6Qin ZK, Yang JA, Ye YL, et al. Expression of Bmi - 1 is a prognostic marker in bladder cancer[J].BMC Cancer, 2009,9:61. 被引量:1
  • 7Breuer RH, Snijders PJ, Sutedja GT, et al. Expression of the p16INK4a gene product, methylation of the p16INK4a promoter region and expression of the polycomb - group gene BMI - 1 in squamous cell lung carcinoma and premalignant endo - bronchial lesions [ J ]. Lung Cancer ;2005,48 ( 3 ) : 299 - 306. 被引量:1
  • 8Silva J, Garcia JM, Pena C, et al. Implication of poly- comb members Bmi -1 ,Mel- 18, and Hpc-2 in the regulation of p16INK4a, p14ARt, h- TERT, and c- Myc expression in primary breast carcinomas [ J ]. Clin Cancer Res, 2006,12(23) :6929 -6936. 被引量:1
  • 9Dhawan S, Tschen SI, Bhushan A. Bmi - 1 regulates the Ink4a/A9c locus to control pancreatic β - cell proliferation [J]. Genes Dev ,2009 ,23 (8) : 906 -911. 被引量:1
  • 10刘建化,黄开红,李学先,宋立兵,郭宝红,冯艳,曾木圣.胃癌患者预后及病理因素与Bmi-1表达的相关性研究[J].中国病理生理杂志,2007,23(10):2018-2022. 被引量:4

共引文献8

同被引文献51

  • 1Liu S, Dontu G,,Wieha MS. Mammary stem cells, self-renewal Path- ways, and eareinogenesis [J].Breast Cancer Res,2005:7(3):8-95. 被引量:1
  • 2Visapaa H, Swligson D, Eeva M, et al. 8q24 amplification intransitional cell carcinoma of bladder [J]. Appl Immunohistochem Mol Morpho, 1 2003, 11(1): 33-36. 被引量:1
  • 3Jourdan F, Sebbaghn, COmperate,et al. Tissuemieroarray technology: validation in eoloreetal carcinoma and analysisofp53, hM-LH1, and hMSH2 immunohistochemical expression [J]. Virehows Arch, 2003, 443(2): 115-121. 被引量:1
  • 4Dhar DK, Udagawa J, Ishihara S, et al. Expression of regenerating gene 1 in gastric adenocareinomas: Correlation with tumor differentiation status and patient survival [J].Cancer,2004,100(6): 1130-1236. 被引量:1
  • 5Liu S,Dontu G,Wicha MS.Mammary stem cells,self-renewal pathways,and carcinogenesis[J].Breast Caneer Res,2005,7(3):8-95. 被引量:1
  • 6Visapaa H,Swligson D,Eeva M,et al.8q24amplification intransitional cell carcinoma of bladder[J].Appl Immunohistochem Mol Morphol,2003,11(1):33-36. 被引量:1
  • 7Jourdan F,Sebbagh N,Comperat E,et al.Tissue microarray technology:validation in colorectal carcinoma and analysis of p53,hMLH1,and hMSH2immunohistochemical expression[J].Virchows Arch,2003,443(2):115-121. 被引量:1
  • 8Weidner N,Semple JP,Welch WR,et al.Tumor angiogencsis and metatasis-correlation in invasive breast carcinoma[J].N Engl J Med,1991,324(1):1-8. 被引量:1
  • 9Dhar DK,Udagawa J,Ishihara S,et al.Expression of regenerating gene 1in gastric adenocareinomas:correlation with tumor differentiation status and patient survival[J].Cancer,2004,100(6):1130-1136. 被引量:1
  • 10Liu S, Dontu G, Wieha MS. Mammary stem ceils, self - renewal Pathways, and eareinogenesis [ J ]. Breast Caneer Res, 2005, 7 (3): 86-95. 被引量:1

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