摘要
目的探讨一种新的人工合成CK2选择性抑制剂四溴肉桂(TBCA),对前列腺癌细胞增殖及周期的影响。方法 CK2选择性抑制剂TBCA应用到多种前列腺癌细胞系,Alamar blue法和克隆形成实验检测细胞生长和增殖能力,流式细胞技术检测细胞周期分布,治疗组与对照组间差异是否具有显著意义采用SPSS统计软件进行分析。结果低剂量(<25μmol)TBCA干预下未发现细胞聚集和分离,而在高剂量(>50μmol)则可观察到细胞明显分离,剂量依赖性抑制细胞生长和增殖(P<0.05),半抑制浓度值(IC50)为25μmol。TBCA诱导前列腺癌细胞停滞在G2/M期细胞周期。结论 TBCA呈剂量依赖性抑制前列腺癌细胞增殖和细胞周期停滞在G2/M期。
Objective To investigate the role of Casein kinase 2 （CK2）-selective CK2 inhibitor Tetrabromocinnamic acid （TBCA） in cell proliferation and cell cycle in prostate cancer cell lines,and to explore a new chemotherapy for prostate cancer. Methods Prostate cancer cell lines were prepared. With TBCA treatment,Alamar-blue assay and clone formation assay were performed to assess cell proliferation and viability. Flow cytometry was performed for cell cycle analysis. The mean and standard error of the mean from Alamar-blue were shown. Results With TBCA treatment,Alamar-blue reading decreased in a dose-dependent fashion,and the IC50 was around 25 μmol. Significant G2/M arrest was detected in prostate cancer cells. As observed under microscope,there were no obvious cell round-up and detachment from the culture surface at a low dose （〈 25 μmol） of TBCA,while cell detachment became obvious at a high dose （〉50 μmol）. Oonclusions The selective CK2 inhibitor TBCA dramatically inhibited cell proliferation and caused a G2/M phase arrest in prostate cancer cells.
出处
《现代泌尿外科杂志》
CAS
2013年第6期542-545,共4页
Journal of Modern Urology
基金
国家自然科学基金(No:81302224)
关键词
酪蛋白激酶2
前列腺癌
四溴肉桂酸
细胞增殖
雄激素受体
Casein kinase 2
prostate cancer
Tetrabromocinnamic acid
cell proliferation
androgen receptor
