摘要
目的 :通过动物实验探讨预防硬膜外粘连的有效材料和方法。方法 :30只大白兔切除L1、L3、L5椎板造成 10×5mm硬脊膜裸露区后随机分为 5组 ,A组用纯化自体脂肪颗粒加碱性成纤维细胞生长因子 (bFGF)覆盖 ,B组用经胰岛素液浸泡的纯化自体脂肪颗粒覆盖 ,C组用未纯化自体脂肪颗粒覆盖 ,D组用 2 %透明质酸钠覆盖 ,E组不用任何间置物覆盖。术后 2、4、6、8、12周进行肉眼及光镜观察 ,并进行硬膜粘连程度分级。结果 :术后 12周时B、C、D、E组均形成了致密粘连 ,只A组无粘连发生。结论 :纯化自体脂肪颗粒加bFGF为预防椎管内粘连的最有效方法。
Objective:To find out effective substances and methods for the prevention of peridural adhesion.Methods:Laminectomy was performed in lumbar 1,3 and 5 segment in 30 adult rabbits which were divided into 5 groups. A 10mm×5mm dura was exposed, and covered with purified autogenous fat particle mixed with bFGF in gourp A, with purified autogenous fat particle treated with insulin in group B, with unpurified autogenous fat particle in group C, with 2% SHA in group D, and uncovered in group E. The specimens were observed grossly and histologically at 2,4,6,8 and 12 weeks postoperatively.Result:Severe peridural adhesion were formed in group B,C,D,E after laminectomy, and no adhesion was formed in group A.Conclusion:Purified autogenous fat particle mixed with bFGF has the best effect in prevention peridural adhesion after laminectomy.
出处
《中国脊柱脊髓杂志》
CAS
CSCD
2000年第6期344-347,共4页
Chinese Journal of Spine and Spinal Cord
关键词
椎板切除术
硬膜
自体脂肪颗粒
透明质酸钠
Laminectomy
dura
adhesion
autogenous fat particle
bFGF
SHA
Rabbit
