摘要
Experiments were conducted to obtain the values of the Sauter bubble size, enrichment and recovery of bovine serum albumin （BSA） in a semi-batch col- umn fitted with a stainless steel sparger at elevated pressure. The effects of Sur- face tension, surfactant concentration, foam/solution height ratio and air flow rate on the separation performance were investigated, and the results showed that good en- richments and recoveries can be achieved for bovine serum albumin operated at el- evated pressures. Especially the size of bubbles generated by the stainless steel sparger was smaller at higher pressures which is favorable to the foam separation process. Furthermore, the separation mechanism of bovine serum albumin operated at elevated pressure was also discussed.
以不锈钢筛板为气体分布器的半连续泡沫分离中,研究压力对气泡尺寸分布和牛血清蛋白富集比和回收率的影响,考察了表面张力、表面活性剂浓度、泡沫/液高度比和气体流速对分离效果的影响。结果表明,在加压条件下牛血清蛋白分离效果有所提高。随着泡沫分离塔内液相表面压力的增加,气泡平均直径逐渐减小;并且随着液相表面压力的增加,牛血清蛋白的富集比逐渐增加。最后,该研究对压力影响牛血清白蛋白分离机理进行了解释。
