摘要
目的评价小檗碱(BBR)对预防家兔动脉粥样硬化发生及斑块破裂、血栓形成疗效,并探讨其可能的作用机制。方法选用新西兰大白兔40只,采用球囊损伤+高脂饮食+药物触发综合的方法建立动脉粥样硬化模型。随机分4组:普通饲料喂养、单纯球囊损伤+高脂、球囊损伤+高脂+小檗碱干预和球囊损伤+高脂+辛伐他汀干预。检测不同时期血脂水平及药物触发前血清ox-LDL、Lp-PLA2、MCP-1、MMP-9、TIMP-1水平。观察斑块破裂和血栓形成情况;并苏木素—伊红(HE)染色,利用微机图像处理测量系统对切片进行图像摄取和转换,测量病变内、中膜的厚度。结果小檗碱可显著降低高脂饮食兔血清TC、TG、LDL-C水平,显著降低血清ox-LDL、Lp-PLA2、MCP-1、MMP-9水平,升高血清TIMP-1水平,与他汀干预组无显著差异。小檗碱组弹力纤维破坏不明显,仍呈连续排列,内膜也有局限性轻度增厚,中膜变薄,内膜厚度小于中膜厚度。小檗碱组的内膜增生程度小于阳性对照组,中膜也没有阳性对照组萎缩明显,和辛伐他汀组的内膜增生程度和中膜萎缩程度近似,药物激发无一例发生斑块破裂与血栓形成。结论小檗碱可显著改善AS病变程度及稳定斑块,作用效果与辛伐他汀相似,其机制可能与小檗碱能显著降低血清及血清各炎症标志物水平等作用有关。
Objective To observe effect of Bererine on regressing atherosclerotic plaques, and to investigate its possible mechanisms. Methods Forty New Zealand White Rabbits were randomly divided into four groups（10/group）. Blood samples were collected in different periods to measure lipid profile and to quantify the plasma concentration of different inflammation mediators. The aorta was dissected and excised to observe the occurrence of plaque rupture and thrombosis. The abdomifial aorta was processed and examined by hematoxylin and eosin staining. Histopathological slides were analyzed by use of a computer-assisted morphometric analysis system , the intima and Tunica media vasorum thickness were measured . Results Nine rabbits in group 1 ,2 and group 3,and 8 rabbits in group 4 completed the study. Plaque rupture and thrombosis occurred in five rabbits from the remaining nine rabbits in group 2 after pharmacological triggering,while there were no plaque rupture in the other three groups. Serum TC ,TG,LDL-c and inflammation mediators levels of Berberine group and simvastatin group was decreased compared with high-cholesterol group（ P 〈 0.05 ） , and they were both much higher than those of normal diet group. In contrast, no significant differences were found between Berberine group and simvastatin group. Pathologic staining demonstrated that the intima was thin and complete in group 1. A great quantity of widespread fatty plaque were seen in group 2, in which intima thickened and foam cell accumulated obviously. In contrast, in the berberne prevention group and the simvastatin control group, plaque thickness diminished and both quantity and volume of foam cell decreased. The intima and Tunica media vasorum thickness in group 2 were significantly higher than the corresponding values in the berberue prevention group or the simvastatin control group. The I/ M of artery in the group 2 was higher than that in the berberine prevention group or simvastatin control group （P 〈 0.01 ）. There was no significant differen
出处
《安徽医药》
CAS
2013年第11期1849-1852,共4页
Anhui Medical and Pharmaceutical Journal
关键词
小檗碱
兔
动脉粥样硬化
炎症标志物
berberine
rabbits
atheroselerosis
markers of infammation
