摘要
随着单精子及精子细胞卵胞浆内显微受精技术的发展,雄性生殖细胞冷冻保存技术已成为辅助生殖技术的重要内容,为男性因素不育患者的临床助孕提供了物质保障基础。目前,雄性生殖细胞冷冻方法包括程序化冷冻和玻璃化冷冻;冷冻保护剂可有效降低细胞的冷冻损伤,根据其特性分为渗透性保护剂和非渗透性保护剂两大类;冷冻载体中冷冻环、冷冻叶片等应用较广泛。雄性生殖细胞冷冻的效果与冷冻方法、冷冻保护剂和冷冻载体等密切相关。
Following the development of intracytoplasmic sperm and haploid spermatid injection techniques,the cryopreservation technology of male germ cell, as important part of assisted reproductive technology （ART）, has played a key role in the ART treatment for the couples with male infertility. Freezing methods of male germ cells include the programmed cryopreservation and vitrification freezing. Cryoprotectant , usually classified as permeable and impermeable cryoprotective agents,could minimize the cell injuries during the freeze-thaw. Various frozen carriers such as cryoloop and cryoleaf are widely used in cell cryopreservation. In this paper,the freezing method,carrier,cryoprotectants and frozen effect of male germ cell were reviewed.
出处
《国际生殖健康／计划生育杂志》
CAS
2013年第6期478-480,共3页
Journal of International Reproductive Health/Family Planning
关键词
低温保存
精液保存
精子
精细胞
精子注射
细胞质内
玻璃化冷冻
冷冻载体
冷冻保护剂
Cryopreservation
Semen preservation
Spermatozoa
Spermatids
Sperm injections,intracytoplasmic
Vitrification
Frozen carrier
Cryoprotectant
