摘要
目的:观察骨癌痛小鼠背根神经节(dorsal root ganglion,DRG)中趋化因子CXCL1和受体CXCR2的表达变化。方法:单侧股骨内注射前列腺癌细胞RM-1诱导小鼠产生骨癌,用行为学检测小鼠抬爪次数和缩爪时间自发痛情况,用实时定量PCR和免疫荧光染色技术观察小鼠DRG中CXCL1和CXCR2的表达变化和细胞定位。结果:骨癌组小鼠的抬爪次数和缩爪时间,从术后10 d开始均较对照组明显上升(P<0.05),并持续到21 d以上。骨癌组小鼠同侧L3-5 DRG中CXCL1和CXCR2 mRNA在手术后7 d时表达较正常小鼠明显增加(P<0.05),并能持续到21 d以上。CXCL1和CXCR2表达于DRG神经元内,骨癌组同侧L3-4 DRG中CXCL1和CXCR2阳性神经元在手术后7,14 d和21 d时较正常组明显增多(P<0.05)。结论:骨癌小鼠DRG中CXCL1和CXCR2表达增加,DRG中的CXCL1及其受体CXCR2可能参与骨癌痛的调节。
Objective： To observe the expression change of CXCL1 and CXCR2 in the dorsal root ganglion （DRG） of mice with bone cancer pain. Methods： Bone cancer pain was induced by injection of prostate cancer cells RM-1 into the unilateral femur of mice. The flinching and guarding behaviors were tested to assess the spontaneous pain. The mRNA expression and the distribution of CXCL1 and CXCR2 were observed by Real-time PCR and immunofluorescence respectively. Results： The frequency of flinching and duration of guarding increased significantly, starting from 10 days until more than 21 days after surgery in bone cancer group compared with naive group （ P 〈 0.05 ）. The upregulation of CXCL1 and CXCR2 mRNA expression in L3-5 DRG was significantly increased at 7 days after surgery in bone cancer group compared with ha＇lye group （P 〈 0.05 ） , and lasted for more than 21 days after surgery. The protein of CXCL1 and CXCR2 was located in DRG neurons. The CXCL1- and CXCR2-immunoreactive neurons were increased at 7, 14 days and 21 days after surgery in bone cancer group compared with na＇fve group （P 〈 0.05 ）. Conclusion： The expression of CXCL1 and CXCR2 in the DRG was upregulated in the mouse model of bone cancer. The CXCLI-CXCR2 signaling in theDRG may be involved in regulating the bone cancer pain.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2013年第6期603-608,共6页
Chinese Journal of Neuroanatomy
基金
江苏省自然科学基金(BK2010273)
南通市科技计划项目(HS12926)
南通大学研究生科技创新计划项目(YKC12040)
江苏高校优势学科建设工程
