摘要
旨在通过优化还原型谷胱甘肽的发酵及分离纯化条件,为GSH大规模的工厂化生产提供理论基础。首先通过筛选获得一株GSH高产菌酿酒酵母Y18,其胞内GSH含量为9.05 mg/g干菌体。进一步研究表明,在培养基中添加L-半胱氨酸和缬氨酸,以及发酵过程中补加葡萄糖可使GSH的总产量分别提高62.0%和146.1%。此外,对GSH在水溶液中稳定性的研究发现偏酸性环境(pH4.5)和氮气保护可显著降低GSH的损失率。最终通过对大孔树脂D001×7和Sephadex G-10的分离纯化条件的优化,获得纯度为98.1%的GSH,收率为73.2%。
The aim of the study is to provide theoretical basis in GSH large-scale production, by optimizing the conditions of its fermentation and purification. Firstly, a GSH high-yield Saccharomyces cerevisiae Y18 was obtained, with the production of 9.05 mg GSH per gram dry cells. Secondly, our results indicated that adding L-cysteine and Valine to the medium, or additional glucose during fermentation process could increase GSH production by 62.0% and 146.1%, respectively. Moreover, the weak acidity condition （ pH4.5 ） and nitrogen input protected GSH from oxidation, and improved its stability. Finally, we obtained high-purity （ 98.1% ） GSH and a promising recovery （ 73.2% ） after optimizing the conditions for separation and purification GSH in Macroporous resin D001 x 7 and Sephadex G-10 gel chromatography.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第11期180-185,共6页
Biotechnology Bulletin
关键词
酿酒酵母
还原型谷胱甘肽
发酵
分离纯化
Saccharomyces cerevisiae Reduced glutathione Fermentation Separation and purification
