摘要
本文报道了新型辐射增敏药甲硝唑氨酸(CM)及其与Ca^(+2)结合后对肝癌H_(22)细胞DNase作用,并观察了CM,CMCa及Ca^(+2)和三者分别对受30Gyγ线照射细胞DNase活力的影响。结果表明,CM浓度在10mmol/L以下对H_(22)细胞DNase有激活作用。以5mmol/L时激活作用最强,在5mmol/L以下随药物浓度的增加,激活作用随之增加,CM与Ca^(+2)结合成CMCa后对细胞DNase的激活作用与CM相似,两者无显著差别(p>0.05),CM、CMCa的这种激活作用与γ线照射后对DNase活力增高有叠加作用,而Ca^(+2)对受照或非受照H_(22)细胞DNase活力无明显的影响。上述结果表明,CM及其与Ca^(+2)结合成CMCa后无论对受照或非受照H_(22)细胞DNase均有激活作用。这种通过激活受照肿瘤细胞DNase叠加活力的增加、增强DNA破坏而提高肿瘤放疗效果,可能是其放疗作用的机制之一。
The effects of a new radiosensitizing compound-metronidazolamic acid(CM) and its Ca^(+2) chelation compound (CMCa) on the DNase activity of mouse he-patic tumor (H_(22)) cells were observed. The effects of CM, CMCa or Ca^(+2) at same mo-larity and their combination with γ-rays irradiation on DNase activity of H_(22) cellswere also observed respectively. The results showed that CM could activate the DNaseactivity to be increased as CM concentratiom increased below 5 mmol/L. The highestDNase activity was activated by CM at 5 mmol/L. The degree of activation betweenCM and CMCa had no significant differente (p>0.05), whereas free Ca^(+2) alone hadno effect. The activation of combined CM or CMCa and radiation groups were greaterthan that of each given independently (Fig. 3), they possessed a coordinate activation.However free Ca^(+2) still could not activate the DNase activity after γ-irradiation. Fromall the above results, it is suggested that CM or CMCa improved the tumor radiothe-rapeutic efficiency probable due to its activation the DNase activity of cancer cellswhich enhanced the DNA damage. It might be one of the radiosensitizing mechanismin tumor radiothorapy.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
1991年第2期65-68,共4页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金
上海市青年科学基金
关键词
辐射增敏剂
甲硝唑氨酸
H22细胞
Radiosensitizer
Metronidazolamic acid
H_(22) cells
DNase
