摘要
目的 揭示锰超氧化物歧化酶（MnSOD）在H2O2诱导PC12细胞凋亡中的作用及机制。方法 转染人正义和反义MnSOD基因的PC12细胞，用50 (mol H2O2攻击已转染的细胞系，并采用MTT法、流式细胞术和 Hoechst染色方法研究H2O2的细胞毒作用。结果 转染正义MnSOD的细胞比原细胞的MnSOD酶活性提高1.3倍，而转染反义MnSOD细胞的酶活性降低67%。结论 H2O2对PC12细胞毒作用是通过诱发PC12细胞凋亡作用来实现的；MnSOD具有抑制H2O2诱导PC12细胞凋亡作用，涉及线粒体的损伤，导致氧化还原失衡继而诱发细胞凋亡。
Objective To explore the effect and mechanism of manganese superoxide dismutase (MnSOD): on H2O2-induced apoptosis of PC12 cells. Methods PC12 cells were transfected with sense and antisense human MnSOD by lipofectin, and exposed to 50 (mol/L H2O2, MTT assay, low cytometry and Hoechst stain were used to study the cytotoxicity. Results MnSOD activity increased by 130% in cells transfected with sense MnSOD cDNA, and was decreased by 67% in cells transfected with antisense MnSOD cDNA as compared with controls. Conclusion Antisense MnSOD can offer protection against H2O2-induced apoptosis in PC12 cells. The damage of the mitochondria may be attributed to the cytotoxicity of H2O2 against PC12 cells, which induces imbalance of cellular redox and apoptosis.
出处
《第一军医大学学报》
CSCD
2000年第6期510-512,F003,共4页
Journal of First Military Medical University
