摘要
根据GenBank已登陆的SRBSDV S10核苷酸序列设计引物,克隆SRBSDV江西分离物外壳蛋白(CP)基因,结合已公布的序列,通过生物信息学方法进行序列分析。结果表明,SRBSDV江西分离物CP基因全长1 674个核苷酸,推测编码557个氨基酸,与已报道的SRBSDV CP基因之间核苷酸同源性为97.5%～100%,氨基酸同源性为97.7%～100%。SRBSDV CP基因核苷酸序列高度保守,保守位点占全部位点的83.5%。没有发现重组事件。本研究首次报道根据系统发育进化树,SRBSDV可分为2大组群,不存在明显的地域和寄主分化。
According to the nucleotide sequences of SRBSDV S10 from GenBank,primers were designed.By reverse-transcription polymerase chain reaction （RT-PCR） and sequencing techniques,the S10 gene of SRBSDV isolated from Jiangxi was cloned and sequenced,and the obtained sequence,together with partial corresponding sequences available from GenBank,were used for molecular variation analysis by using some bioinformatics softwares.The result indicated that the S10 gene of SRBSDV isolated from Jiangxi was consisted of 1785nts,including 1 674 nucleic acids of full length CP gene,encoding 557 amino acids.The CP gene nucleotide and amino acid sequences homology of SRBSDV Jiangxi isolate and other isolates in GenBank were between 97.5％-100％ and 97.7％-100％,respectively.SRBSDV CP gene nucleotide sequences were highly conservative.No recombination event was detected.Phylogenetic analysis suggests that SRBSDV isolates could be divided into two clusters,but there was no significant correlation between clusters and geographic origin or host.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2013年第5期901-905,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家"十二五"科技支撑计划课题(2012BAD14B1)
江西省重大科技专项计划(20114ABF03102)
高等学校博士学科点专项科研基金(20113603110001)
国家转基因生物新品种培育重大专项(2011ZX08001-002)
关键词
南方水稻黑条矮缩病毒
CP基因
基因克隆
序列分析
Southern rice black-streaked dwarf virus （SRBSDV）
coat protein gene
gene cloning sequence analysis
