摘要
目的研究非酒精性脂肪肝(nonalcoholic fatty liver disease,NAFLD)的环境危险因素、相关基因的遗传多态性以及基因与环境因素的交互作用,为NAFLD的早期诊断、早期预防提供理论依据。方法采用病例对照的方法,对324例汉族NAFLD患者(NAFLD组)及304例汉族健康人(对照组)进行回顾性问卷调查,利用基因芯片技术测定载脂蛋白B(ApoB)的基因多态性。结果 (1)WHR(腰臀比)、男性和高脂血症病史为NAFLD的独立危险因素,而适当的活动量则为其保护因素。(2)病例组ApoB基因的11039G/A多态性和7673C/T多态性基因型频率及等位基因频率和对照组分布差异具有统计学意义。(3)ApoB11039G/A多态性GA基因型与基本不活动具有相乘交互作用,OR值为5.16。ApoB7673C/T多态性CT和TT基因型则与WHR和高脂血症病史均具有明显的相乘交互作用,OR值分别为14.43和14.95。结论 ApoB11039G/A多态性和7673C/T多态性与NAFLD的发病有关联,ApoB11039G/A多态性和ApoB7673C/T多态性与WHR、高脂血症病史和多坐少动的生活节奏等环境因素存在很强的交互作用。在人群中开展ApoB基因多态性的检测,对筛选出的脂肪肝高危人群加强卫生宣传教育和行为干预具有重要意义。
Objective To explore the environmental risk factors, gene polymorphisms in nonalcoholic fatty liver disease （NAFLD） and their interaction for early diagnosis and prevention of NAFLD. Methods A retrospective case-control study was used to explore the related risk factors of NAFLD for analysis. Genet- ic polymorphisms of apolipoprotein B were determined by the technique of DNA chips. Results （1） WHR, male and hyperlipidemia were positively correlated to the occurrence of NAFLD,while some daily activities were negatively correlated to the occurrence of NAFLD. （2） Significant differences were found in distribution of frequencies of polymorphisms in the ApoB11039G/A and ApoB7673C/T between NAFLD group and control group. （3） In ApoB7673C/T polymorphism, CT and TT genotype had shown a signifi- cant gene-environment interaction with both WHR and hyperlipaemia in the occurrence of NAFLD. Con- clusion ApoBl1039G/A and 7673C/T polymorphism were associated with the occurrence of NAFLD. The study showed significant interaction between inherited factors and environmental factors in NAFLD. Therefore, the polymorphisms of ApoB 11039G/A and 7673C/T should be determinate to screen suscep tire persons for early diagnosis and prevention.
出处
《新疆医科大学学报》
CAS
2013年第11期1654-1657,共4页
Journal of Xinjiang Medical University
基金
国家重点基础研究发展计划(973计划)(2012CB722403)
关键词
非酒精性脂肪肝
遗传
环境
交互作用
nonalcoholic fatty liver disease
genic polymorphism
environment
interaction
