摘要
目的以宠物犬为研究对象,监测在自然感染条件下其体内乙型脑炎病毒(JEV)中和抗体随流行季节的变化规律,进而评估JEV感染人的风险性。方法按月采集不同年龄的宠物犬血液,分离血清,用微量细胞中和试验检测血清JEV中和抗体。结果共得到宠物犬血清253份,中和抗体阳性率达到28.1%。不同季节间犬JEV中和抗体阳性率差异显著,JEV流行季节(6-9月)之前(3-5月)和之后(10-12月)的3个月中和抗体阳性率分别为16.3%和43.4%,差异有统计学意义(χ2=14.432,P<0.01)。小于1岁与大于1岁的宠物犬中和抗体阳性率分别为24.1%和39.0%,二者差异显著(χ2=5.633,P<0.05)。而犬的性别间中和抗体阳性率显著无统计学意义(χ2=1.860,P>0.05)。结论在JE流行季节宠物犬受到JEV自然感染的机率较高,可作为哨兵动物来评估JEV感染人的风险。
In this experiment, we collected 253 samples of sera from companion dogs with different ages during 2011- 2012 and detected JEV neutralizing antibodies. The overall positive rate of JEV neutralizing antibodies in companion dogs was 28.1%, with significant seasonal variation. The positive rate for the months before （March to May） and after （October to De- cember） JE epidemic season （June to September） was 16. 3% and 43. 4%, respectively, showing a statistically significant difference between the two groups （X2 =14. 432, P〉0.01）. The positive rate for dogs younger and older than one year was 24.1% and 36.1%, respectively, and a statistically significant difference was detected between the two groups （3（2 =3. 952, P d0.05）. The positive rate for the male and female companion dogs was 32.5% and 24.6%, respectively, while no statistical significance was found （Xz= 1. 860, P〉0.05）. Results showed that companion dogs were infected with JEV seriously, espe cially in the JE epidemic season, and companion dogs could serve as a sentinel animal to assess the risk of human infection by JEV
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2013年第11期1050-1054,共5页
Chinese Journal of Zoonoses
基金
国家国际科技合作项目(No.2010DFB33920)
国家质检总局科技计划项目(No.2012KJ)联合资助~~
关键词
乙型脑炎
宠物犬
流行病学调查
中和抗体
哨兵动物
Japanese encephalitis
companion dogs
serosurvey
neutralizing antibody
sentinel animal
