摘要
目的:采用高效液相色谱法建立复方土荆皮酊的指纹图谱。方法:采用Agilent Zorbax SB C18(4.6 mm×250 mm,5μm)色谱柱,以甲醇(B)-0.09%甲酸水溶液(A)为流动相进行梯度洗脱,检测波长260 nm,流速1.0 mL·min-1,柱温30℃。采用国家药典委员会颁发的《中药色谱指纹图谱相似度评价系统》(2004 A版)软件,对14批次复方土荆皮酊的指纹图谱进行相似度计算。结果:根据14批次复方土荆皮酊的指纹图谱,确定了13个共有指纹峰,其相似度均>0.960,建立了对照指纹图谱。结论:该方法的精密度、重复性、稳定性良好,为复方土荆皮酊的质量控制提供了科学依据。
Objective：To establish a fingerprint of Fufang Tujingpiding by HPLC.Method：The experiment was conducted on Agilent Zorbax SB C18 （4.6 mm × 250 mm,5 μm）,eluted with methanol （B）-0.09% formic acid aqueous solution （A） as the gradient elution mobile phases.The detective wavelength was set at 260 nm with the flow rate of 1.0 mL ·min-1 The column temperature was maintained at 30 ℃.The data was analyzed by Similarity Evaluation Software for Chromatographic Fingerprint of Traditional Chinese Medicine software to compare the quality of 14 batches of Fufang Tujingpiding.Result：The standard chromatographic fingerprints of Fufang Tujingpiding was established,13 co-possessing peaks were marked according to the fingerprints of 14 batches of it,and the similarity was greater than =.96.Conclusion：The established method has good precision,reproducibility and stability,which provides the basis for the quality control of Fufang Tujingpiding as well.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第22期134-136,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
军队"十二五"规划项目(军队医疗机构制剂标准提高研究课题)
