摘要
目的:研究注射用益气复脉(冻干)碱醇洗脱部分的最佳甲酯化方法。方法:分别采用1%硫酸-甲醇加热回流酯化法,10%硫酸-甲醇加热回流酯化法,25%硫酸-甲醇加热回流酯化法和2%氢氧化钾-甲醇加热回流酯化法对该部分物质进行甲酯化,并用合适的溶剂进行萃取,之后将所得萃取物进行GC-MS分析。结果:当采用10%硫酸-甲醇加热回流酯化法对该部分物质进行甲酯化并选用氯仿做萃取剂时,酯化效率最高。结论:10%硫酸-甲醇加热回流酯化法是注射用益气复脉(冻干)碱醇洗脱部分的最佳甲酯化方法,为该部分主要成分的研究提供依据。
Objective：To study the optimal methyl esterification method in alkali alcohol eluent from Yiqi Fumai lyophilized injection.Method：Some esterification methods were adopted as follows：1% sulfuric acid-methanol refluxed esterification method,10% sulfuric acid-methanol refluxed esterification method,25%sulfuric acid-methanol refluxed esterification method and 2% potassium hydroxide-methanol refluxed esterification method.The solution of methyl esterextraction was extracted by opportune solvent,then,analysis them with GCMS.Result：10% sulfuric acid-methanol refluxed esterification with extracted by chloroform is the optical method,which shows the best esterification efficiency.Conclusion：10% sulfuric acid-methanol refluxed esterification is the best esterification method of alkali alcohol eluent from Yiqi Fumai lyophilized injection,which provides the basis for the research on the main ingredients of this extract.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第22期96-99,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
科技重大专项"重大新药创制"项目(2010ZX09502-004)
