摘要
目的:评价新疆鼠尾草根中6,7-去氢罗列酮的体外抗血小板聚集作用并优选其提取工艺。方法:应用比浊法测定6,7-去氢罗列酮体外抗血小板聚集作用。在单因素试验基础上,以6,7-去氢罗列酮提取率为指标,通过正交设计法考察提取次数、料液比和提取时间对6,7-去氢罗列酮提取工艺的影响。采用HPLC测定6,7-去氢罗列酮含量,色谱条件为依利特C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.02%甲酸(83∶17),流速1.0 mL·min-1,检测波长330 nm,柱温40℃。结果:6,7-去氢罗列酮高、中剂量组抑制凝血酶诱导的血小板聚集作用与生理盐水组比较均具有极显著性差异,高剂量组在15,30,45,60 min内由凝血酶诱导的血小板抑制聚集率分别为90%,75%,66%,64%。最佳提取工艺为加10倍量95%于85℃提取3次,每次3 h,新疆鼠尾草根中6,7-去氢罗列酮质量分数0.01%。结论:新疆鼠尾草根中6,7-去氢罗列酮表现出较强的抗血小板聚集作用,在此活性基础上优选的提取条件稳定可行。
Objective： To evaluate anti-platelet aggregation of 6, 7-dehydroroyleanone in roots of Salvia deserta and optimize its extraction process. Method： In vitro anti-platelet aggregation of 6, 7-dehydroroyleanone was determined by turbidimetry. Based on single factor tests, orthogonal test was adopted to effects of extracting times, solid-liquid ratio, extracting time on extraction technology of 6, 7-dehydroroyleanone with yield of 6, 7- dehydroroyleanone as index. The content of 6, 7-dehydroroyleanone was determined by HPLC with mobile phase of methanol-0.02% formic acid （ 83 ： 17 ） , detection wavelength of 272 nm, column temperature at 40 ~C. Result： By comparing with the saline group, the high and middle dose group of 6, 7-dehydroroyleanone could effectively inhibit platelet aggregation induced by thrombin, platelet aggregation inhibition rates of the high dose group of 6, 7-dehydroroyleanone within 15, 30, 45, 60 min were 90%, 75%, 66%, 64%, respectively. Optimum extraction process was as following： extracted 3 times with ten-fold the amount of 95% ethanol at 85 ~C for 3 h each time ; The content of 6, 7-dehydroroyleanone in roots of S. deserta was 0.01%. Conclusion ： 6, 7- Dehydroroyleanone in roots of S. deserta showed better anti-platelet aggregation effect, optimized extraction technology was stable, reasonable and feasible which based on the activity.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第22期15-18,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部"十一五"支撑计划项目(2007BAI30B02-2)
新疆维吾尔自治区高校科研计划科学研究重点项目(XJEDU2012I23)
关键词
新疆鼠尾草
6
7-去氢罗列酮
正交试验
提取工艺
凝血酶
抗血小板聚集
单因素试验
Salvia deserta
6, 7-dehydroroyleanone
orthogonal test
extraction technology
thrombin
anti-platelet aggregation
single factor test
