摘要
目的:优选清燥救肺汤的提取工艺。方法:采用L9(34)正交试验设计,以苦杏仁苷、绿原酸、甘草酸含量及得膏率的综合评分为指标,经综合评分法考察乙醇体积分数、提取时间、提取次数对工艺的影响。利用HPLC测定3种指标成分的含量,色谱条件为Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm),检测波长程序(0~50 min,207 nm;50~100 min,237 nm),柱温30℃,流速1.0 mL·min-1,流动相乙腈(A)-0.1%磷酸水溶液(B)梯度洗脱(0~30 min,5%~10%A;30~55 min,10%~20%A;55~80 min,20%~35%A)。结果:清燥救肺汤的最佳提取工艺为加8倍量70%乙醇提取2次,每次2 h。结论:优选的提取工艺稳定、合理、可行,有效成分提取效率高。
Objective： To optimize extraction technology of Qingzao Jiufei decoction by multi-index comprehensive evaluation method. Method： L9 （34） orthogonal design was adopted, with dry extract rate, the contents of amygdalin, chlorogenic acid and glycyrrhizic acid as comprehensive evaluation index, effects of ethanol concentration, decocting time and times on extraction technology were investigated by composite score method. The contents of three index ingredients were determined by HPLC, chromatographic conditions were as follows： Agilent TC-Cls column .（4.6 mm x 250 mm, 5 p^m）, detection wavelength program （0-50 min, 207 nm; 50- 100 min, 237 rim）, column temperature 30 ~C, flow rate 1.0 mL ＂min-1, mobile phase of acetonitrile （A） 0. 1% phosphoric acid （B） gradient elution （0-30 min, 5%-10%A; 30-55 min, 10%-20%A; 55-80 min, 20%-35% A）. Result： Optimal extraction technology was as following： extracted 2 times with 8 times the amount of 70% ethanol for 2 h per time. Conclusion： This optimized extraction technology was stable, feasible and reasonable with high extraction efficiency of active ingredients.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第22期12-15,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家教育部高等学校博士学科点专项科研基金项目(20112133120001)
辽宁省科技厅博士启动基金项目(20101067)
沈阳市科技基金项目(F11-264-1-62)
辽宁省教育厅优秀人才基金项目(LJQ2011102)
关键词
清燥救肺汤
正交试验
苦杏仁苷
绿原酸
甘草酸
得膏率
波长切换法
Qingzao Jiufei decoction
orthogonal test
amygdalin
chlorogenic acid
glycyrrhizic acid
extract yield
wavelength switching method
