摘要
目的探讨不同剂量草甘膦(GLY)对小鼠睾丸支持细胞(sertoli细胞)凋亡及细胞存活率的影响,同时观察雄激素结合蛋白(ABP)及波形蛋白mRNA表达的变化。方法体外原代培养小鼠sertoli细胞,收集细胞并分为正常对照组和不同剂量草甘膦组,草甘膦的浓度分别为60、90、120、150和180 mg/L;各组细胞培养24 h后,倒置显微镜下观察细胞生长情况及形态改变;MTT法检测细胞存活率;Hoechst 33342检测细胞凋亡情况;RT-PCR检测草甘膦对ABP及波形蛋白mRNA表达情况。结果在不同浓度草甘膦作用下,sertoli细胞出现不同程度缩小、脱落、甚至破碎;细胞的增值率明显低于对照组(P<0.01);细胞凋亡指数明显高于对照组(P<0.05);细胞ABP mRNA和波形蛋白mRNA表达与对照组相比均减弱(P<0.05)。结论草甘膦对小鼠sertoli细胞有一定的毒性,能诱导细胞凋亡及抑制细胞增殖,且随草甘膦剂量的增加,有害作用有增加的趋势;同时能抑制ABP和波形蛋白mRNA的表达。
Objective To investigate the effect of different doses of glyphosate on apoptosis and expressions of androgen-binding protein （ABP） and vimentin mRNA in mouse Sertoli cells. Methods Primarily cultured mouse Sertoli cells incubated with different doses of glyphosate （60, 90, 120, 150 and 180 mg/L） for 24 h. The growth and morphological alterations in the cells were observed under inverted microscope, and the cell proliferation rate was evaluated withMTT assay. Hoechst 33342 staining was used to detect cell apoptosis after the treatment, and RT-PCR was performed to examine the changes in the expression of ABP and vimentin mRNAs. Results Sertoli cells exposed to glyphosate showed a reduced cell volume, cell dissociation with occasional cell disruption. The proliferation of the exposed was suppressed with an increased rate of cell apoptosis and lowered expressions of ABP and vimentin mRNAs （P〈0.05）. Conclusion GLY can cause cellular damages, inhibit cell proliferation, induce cell apoptosis, and decrease expression of ABP and vimentin mRNAs in mouse Sertoli cells in vitro.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2013年第11期1709-1712,F0003,共5页
Journal of Southern Medical University
基金
蚌埠医学院科技发展基金重点项目(BYkf12A09)
蚌埠医学院院级课题(BY1021)
关键词
草甘膦
小鼠睾丸支持细胞
雄激素结合蛋白
波形蛋白
glyphosate
sertoli cell of mouse
cell apoptosis
androgen-binding protein
vimentin
