摘要
以水稻优良品种吉农大 8号成熟种子为实验材料 ,建立了悬浮体细胞胚胎发生体系 ,并获得再生植株 .对其发生过程中特异蛋白的变化 ,以及诱导中胚性与非胚性愈伤组织的差异等问题进行了探讨 ,为将来深入研究体细胞胚胎发生机理奠定了基础 .实验结果表明 ,不同培养基上诱导的胚性愈伤组织的发生频率是不同的 .基本培养基NMB诱导形成的愈伤组织在适当激素浓度下 ,可由悬浮培养产生体细胞胚 ,转移到固体培养基上后 ,得到再生植株 .对这一过程进行的组织学观察证实该形态发生途径是单细胞起源的体细胞胚胎发生 .利用SDS -聚丙烯酰胺凝胶电泳技术比较体细胞胚胎发生中不同时期可溶性蛋白组分的差异 ,发现相对分子质量为 590 0 0的蛋白组分存在于分化初期的球形胚中 ;相对分子质量为 560 0 0的蛋白组分仅在早期成熟胚中出现 ;在分化出幼叶的晚期成熟胚中具有相对分子质量为 32 0 0
In this paper, the mature seeds of 'JND-8', the good strain of the rice ( Oryza sativa L.), were induced to produce somatic embryos in suspension culture, and developed into the regeneration plants. Several problems, including the specific-stage proteins occurred during somatic embryogenesis and development, and the difference between embryogenic and non-embryogenic calluses, were discussed in this work.The result showed the frequency of occurrence of the embryogenic calli is different, comparing with different media. The embryogenic calli that are induced on the NMB basic media can develop into the somatic embryos, in suspension culture, by adjusting the concentration of the exogenous hormones. Transformed into the solid differentiation media, these cell suspension cultures can grow into the regeneration plants. From the histological study, the conclusion can be drawn that the somatic embryogenesis of the rice was originated from single cell. The results obtained from the embryogenic and non-embryogenic calluses indicated that these two materials were distinct morphologically. The specific proteins occurring in the different stages were compared by SDS-PAGE technique. There were three proteins (59 000, 56 000, 32 000) found separately in early embryos, mature embryos, and cotyledonary-differentiated stages.
出处
《东北师大学报（自然科学版）》
CAS
CSCD
2000年第4期52-58,共7页
Journal of Northeast Normal University(Natural Science Edition)
基金
国家自然科学基金资助项目(3917039)
关键词
体细胞胚胎发生
特异蛋白
水稻
somatic embryogenesis
specific protein
Oryza sativa L
