摘要
为提高大豆的耐旱耐盐碱性,将拟南芥转录因子DREB2A序列优化后,构建了植物表达载体pCAMBIA3300-DREB2A,利用农杆菌介导法将DREB2A基因导入大豆品种Willimas 82和Bert中,转化率分别为0.8%和3.2%。对抗性转基因植株进行PCR检测,2个品种分别获得阳性植株28和63株,表明DREB2A已整合到大豆基因组中。
To improve the salt and drought tolerance of soybean,a vector of pCAMBIA3300-DREB2A with stress related gene DREB2A from Arabidopsis thaliana was constructed. DREB2A gene was introduced into soybean varieties Williams 82 and Bert by Agrobacterium-mediated transformation. The average transformation frequencies were 0.8% and 3.2% , respectively. Twenty-eight and 63 positive plants respectively were obtained by PCR identification. The results indicated that the gene DREB2A had been integrated into the genome of soybean.
出处
《大豆科学》
CAS
CSCD
北大核心
2013年第5期606-608,共3页
Soybean Science
基金
转基因生物新品种培育重大专项(2011ZX08004-002)
关键词
大豆
DREB2A
耐盐耐旱
农杆菌介导遗传转化
Soybean
DREB2A
Salt and drought tolerance
Agrobacterium-mediated transformation
