摘要
为研究高压脉冲电场辅助处理对总黄酮提取效果的影响,以箬叶为试材,找出了其最佳的处理技术参数。在乙醇浓度、电场强度、脉冲数和料液比4个单因素试验的基础上,采用L9(34)正交试验来优化工艺参数。试验结果表明,料液比是最主要的影响因素,其次分别是电场强度、脉冲数和乙醇浓度。在料液比为1∶40,电场强度为20 kV/cm,脉冲数为8,乙醇浓度为75%的条件下箬叶总黄酮的提取率最高,达到4.89%,比传统提取法提高了2.5倍以上。试验结果表明,高压脉冲电场辅助提取技术在箬叶总黄酮提取工艺中具有较高的应用价值,可作为一种新型工艺技术进行推广。
This study established preliminary conditions for enhancing high intensity pulsed electric fields （HIPEF）-assisted extraction of total flavonoids from Indocalamus tessellatus leaves.Four influencing factors including ethanol concentration （35％,45％,55％,65％,75％,85％,and 95％）,electric field intensity （10,20,30,40,50,60 kV/cm）,electric pulse number （0,2,4,6,8,10 and 12） and ratio of material-solvent （1∶20,1∶30,1∶40,1∶50,1∶60 and 1∶70）were investigated using the L9 （34） orthogonal test.The ratio of material-solvent was the main factor affecting extraction result,followed by electric field intensity,pulse number and ethanol concentration.The optimal conditions were determined as follows：material-solvent ratio of 1 ∶ 40,electric field intensity of 20 kV/cm,pulse number of 8 and ethanol concentration of 75％.Under these conditions,the extraction yield of total flavonoids was 4.89％.HIPEF and conventional extraction method were compared.The extraction yield of flavonoids using the conventional method was 1.56％,and the microwave assisted extraction yield of flavonoids was 1.92％.The results suggested that the HIPEF-assisted extraction process was feasible,and the extraction yield of total flavonoids from I.tessellatus leaves was higher than the conventional method.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2013年第10期1436-1441,1410,共7页
Natural Product Research and Development
基金
2011 Anhui Provincial Natural Science College Research Project(NO.KJ2011B165)
2011 Huangshan University Sciences Research Project(NO.2011xkj003)
关键词
箬叶
总黄酮
高压脉冲电场
辅助提取
Indocalamus tessellatus leaves
total flavonoids
high intensity pulsed electric fields
assisted extraction
