摘要
OBJECTIVE:To investigate the effect of Soothing liver therapy on infertile women undergoing in vitro fertilization and embryo transfer(IVF-ET)and to explore its mechanism.METHODS:Fifty-eight women with tubal infertility were randomized into two groups:30 in an experimental group treated with Xiaoyao powder(Shugan)plus gonadotropin-releasing hormone analog(GnRHa)/follicle-stimulating hormone(FSH)/human chorionic gonadotropin(hCG)and 28 in the control group who were treated with GnRHa/FSH/hCG only.The total gonadotropin(Gn)doses required,endometrial thickness,oocyte numbers,high quality embryo production rate and pregnancy rate of the two groups were compared.The concentration of growth differentiation factor-9(GDF-9)in follicular fluid was detected by western blotting and the expression of GDF-9 mRNA in granulosa cells was measured using reverse tran-scription-polymerase chain reaction amplification.RESULTS:In the experimental group,the Gn dose was significantly lower than that in the control group;the endometrial thickness,high quality embryo production and pregnancy rates were significantly higher and the expression of GDF-9 mRNA was also significantly higher than in the control group(all P<0.05).CONCLUSION:Shugan treatment can improve the pregnancy rate of women with tubal infertility;its mechanism is possibly related to the increased expression of GDF-9 in granulosa cells.
OBJECTIVE： To investigate the effect of Soothing liver therapy on infertile women undergoing in vitro fertilization and embryo transfer（IVF-ET） and to explore its mechanism.METHODS： Fifty-eight women with tubal infertility were randomized into two groups： 30 in an experimental group treated with Xiaoyao powder（Shugan） plus gonadotropin-releasing hormone analog（GnRHa）/follicle-stimulating hormone（FSH）/human chorionic gonadotropin（hCG） and 28 in the control group who were treated with GnRHa/FSH/hCG only. The total gonadotropin（Gn） doses required, endometrial thickness, oocyte numbers,high quality embryo production rate and pregnancy rate of the two groups were compared. The concentration of growth differentiation factor-9（GDF-9） in follicular fluid was detected by western blotting and the expression of GDF-9 mRNA in granulosa cells was measured using reverse tran-scription-polymerase chain reaction amplification.RESULTS： In the experimental group, the Gn dose was significantly lower than that in the control group; the endometrial thickness, high quality embryo production and pregnancy rates were significantly higher and the expression of GDF-9 mRNA was also significantly higher than in the control group（all P0.05）.CONCLUSION： Shugan treatment can improve the pregnancy rate of women with tubal infertility; its mechanism is possibly related to the increased expression of GDF-9 in granulosa cells.
基金
Supported by the National Natural Science Foundation of China(No.81173294)
