摘要
目的探讨医院感染性标本中检出的假丝酵母菌属种类、临床标本分布及对抗真菌药物的敏感性,为临床治疗真菌感染寻求实验室参考依据。方法采用萨布罗培养基培养真菌,用法国生物梅里埃公司生产的API 20C AUX假丝酵母菌属鉴定板条和ATB 3Fungus假丝酵母菌属药敏板条进行假丝酵母菌属的种类鉴定和药敏试验。结果 2011年1月-2012年12月共分离假丝酵母菌属254株,白色假丝酵母菌的分离率居第1位,培养出142株,占总数的55.9%;分离率第2为热带假丝酵母菌,培养出41株,占16.1%;其余依次为光滑假丝酵母菌13.0%、近平滑假丝酵母菌8.7%、克柔假丝酵母菌6.3%,假丝酵母菌属感染部位主要是呼吸道感染,检出率为61.0%;假丝酵母菌属对两性霉素B、氟胞嘧啶、伏立康唑的敏感率最高,敏感率为81.3%～100.0%;而假丝酵母菌属对临床使用较多的药物氟康唑、伊曲康唑的敏感率有降低趋势,克柔假丝酵母菌对氟康唑天然耐药。结论临床医师要提高深部真菌感染的临床治愈率,就必须积极送检感染性标本进行真菌培养,而真菌药敏试验的结果能够指导临床医师合理选择抗真菌药物,避免无序用药导致真菌的耐药性日益升高。
OBJECTIVE To investigate the species of Candida isolated from infectious specimens, distribution of clinical specimen,and the drug susceptibility to antifungal drugs so as to seek laboratory basis for the clinical treatment of antifungal infections. METHODS The Sabouraud＇s agar culture medium was used to culture fungi, the identification of Candida and the drug susceptibility testing were performed by API 20 C AUX strips of BioM6rieux, France and ATB3 FUNGUS strips. RESULTS A total of 254 strains of Candida were isolated from Jan 2011 to Dec 2012, among which 142 （55.9%） strains of Candida albicans isolated ranked the first place, 41 （16.1%） strains of Candida tropicalis the second place, followed by the Candida glabrata （13, 0%）, Candida parapsilosis （8.7 %）, and Candida krusei （6.3 %）. The respiratory tract was the predominant site of Candida infections, the detection rate was 61.0%. The data of the drug susceptibility testing revealed that the Candida were most susceptible to amphotericin B, flucytosine,and voriconazole, with the drug susceptibility rates varying between 81.3% and 100.0% ; however, the drug susceptibility rates to such commonly used antifungal drugs as fluconazole and itraconazole showed a down ward trend, and Candida krusei was naturally resistant to fluconazole. CONCLUSION The infectious specimens should be actively submitted for the fungal culture so as to improve the clinical cure rate of deep fungal infections; the clinicians should reasonably use antibiotics based on the result of drug susceptibility testing so as to avoid the increasing of fungal resistance induced by the drug abuse.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2013年第21期5367-5368,5371,共3页
Chinese Journal of Nosocomiology
