摘要
目的测定人乳铁蛋白转基因山羊奶及非转基因山羊奶中乳糖的含量,考察两者是否存在显著性差异,为人乳铁蛋白转基因山羊奶的实质等同性评价提供依据。方法采用APS-2 HYPERSIL色谱柱,以乙腈-水(7︰3)为流动相,示差折光检测器,测定两种山羊泌乳30 d、60 d及90 d采集的羊奶中各90个样本的乳糖含量,用SPSS软件对测定结果进行统计学处理。结果泌乳30 d、60 d及90 d采集的样本中,转基因山羊奶的平均乳糖含量分别为43.2 mg/ml、37.4 mg/ml及40.2 mg/ml;非转基因山羊奶的平均乳糖含量分别为38.2 mg/ml、31.7 mg/ml及37.2 mg/ml。结论在3个时间段采集的样本中,转基因与非转基因山羊奶中的乳糖含量均不存在显著性差异(P≥0.05)。
Objective To determine the lactose content in human lactoferrin transgenic and non - GMO goats＇ milk, find the difference between the two kinds of goats＇ milk, so as to provide reference for substantial equivalence evaluation on the quality of human lactoferrin transgenie goats＇ milk. Methods The APS - 2 HYPERSIL column was used to determine the lactose content by refractive index detector in 90 samples from both kinds of goats＇ milk, which were collected on the 30th, 60th and 90th lactating day, with the mobile phase of acetonitrile - water （7：3 ）. All the data was analyzed with SPSS software. Results For the samples from the 30th, 60th and 90＇hlactating day, the average values of lactose content in transgenic goats＇ milk were 43.2 mg/ml, 37.4 mg/ml and 40.2 mg/ml respectively, while those in non - GMO goat＇ milk were 38.2 mg/ml, 31.7 mg/ml and 37.2 mg/ml. Conclusion There was no significant difference in lactose content between the two kinds of goats＇ milk from three different lactating days（ P≥0.05 ）.
出处
《中国卫生检验杂志》
北大核心
2013年第12期2610-2612,共3页
Chinese Journal of Health Laboratory Technology
基金
国家农业部转基因生物新品种培育科技重大专项资助项目(2009ZX08011-032B)
