摘要
目的 探讨 p16基因修饰后小鼠肾癌细胞体内致瘤性的变化。 方法 将携带 p16基因腺病毒体外转染的小鼠肾癌Renca细胞 ,p16基因修饰的肾癌细胞、野生型肾癌细胞及对照基因LacZ修饰的肾癌细胞分别接种于小鼠皮下 ,观察肿瘤生长情况及小鼠存活时间 ,每组 10只小鼠。 结果 经p16基因修饰的肾癌细胞在小鼠体内出现肿瘤的时间在Adp16基因实验组比野生型对照组及AdLacZ对照组晚 5天 ,且肿瘤生长缓慢 (P <0 0 5 )。小鼠存活时间 :野生型对照组为 (2 7.0±2 2 )d ,AdLacZ组为 (2 9.0± 2 .5 )d ,Adp16基因实验组为 (5 2 .0± 3 .4)d ,并有 3只小鼠长期存活。肿瘤局部 p16蛋白表达测定显示p16基因已转入肿瘤细胞 ,并在癌体内有效表达。 结论 p16基因的导入能降低小鼠肾癌细胞的体内致瘤性 ,显著延长荷瘤小鼠的存活期。
Objective To investigate the tumorigenicity of p16 transduced renal cancer cells(Renca). Methods Using a 100*!μl syringe and 26-gauge needle,a 100*!μl solution containing 5×10 4 of renal cancer cells (Adp16-Renca,wt-Renca and AdLacZ-Renca respectively) in PBS was slowly injected subcutaneously into the dorsal flanks of BALB/c mice to investigate the tumorigenicity and the survival time of the mice. Results Compared with wt-Renca and LacZ-Renca controls,the duration of tumorigenicity and the survival time of p16-Renca group is much longer. Conclusions Transfer of p16 gene by adenoviral vectors to renal cancer cells can prevent the development of subcutanous tumors and the prolong of survival time of the mice.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2000年第11期653-655,共3页
Chinese Journal of Urology
