摘要
背景角膜上皮损伤可导致角膜溃疡和基质混浊，甚至出现不可逆的视功能损害，以往采用的药物治疗仅能缓解刺激症状，而促进角膜上皮细胞再生的作用较弱，因此寻求能有效调控角膜上皮细胞生长，从而有效治疗角膜上皮损伤的药物至关重要。目的探讨重组人BIGH3蛋白滴眼液对角膜上皮损伤的修复作用。方法50只清洁级健康成年新西兰大白兔，均取右眼为实验眼，其中2只兔制作角膜上皮损伤模型后即刻行裂隙灯显微镜检查及角膜荧光素染色，眼前节照相后处死；48只兔按照随机数字表法随机分为重组人表皮生长因子（EGF）衍生物组（阳性对照组）、生理盐水组（阴性对照组）、质量分数0．25％重组人BIGH3蛋白滴眼液组及O．5％重组人BIGH3蛋白滴眼液组，每组12只。在直径7mm环钻内滴人体积分数20％乙醇，置于角膜中央区60s，然后用角膜刮匙刮去角膜上皮，制作角膜上皮缺损模型。术后各组兔眼分别点用相应药物，每天4次，分别于术后12、24、36、48、72h行裂隙灯显微镜检查及角膜荧光素染色，于12、24、36、48h采用抽签法各处死2只实验兔，72h时各处死4只实验兔，制备角膜组织标本并行苏木精一伊红染色，观察并比较各组兔眼在不同时间点角膜上皮修复情况，应用计算机图像处理系统测量各组兔眼不同时间点角膜上皮愈合面积。结果各组兔眼用药后均未发现任何眼部刺激症状。组织病理学研究发现，造模后即刻全层角膜上皮缺损，随着时间的延长，实验组及对照组兔角膜上皮缺损区皆逐渐变小，可见周边角膜上皮向中央缺损区移行，细胞层数逐渐增加，细胞排列极向逐渐趋于规则。重组人EGF衍生物组、生理盐水组、0．25％及0．5％重组人BIGH3蛋白滴眼液组角膜上皮愈合速率分别为15．00、13．81、18．05、18．86mm。／d。与生理盐水组比�
Background Corneal epithelial abrasion results in corneal ulcer and stroma cloudy even irreversible visual impairment. Previous drugs for corneal epithelial injury can only alleviate the inflammatory irritation. So it is very important to seek a drug which regulate the growth of corneal epithelium. Objective This study was to investigate the effects of recombinant human BIGH3 protein eye drops on corneal epithelial abrasion. Methods Fifty right eyes of 50 clean adult New Zealand white rabbits were collected. Two rabbits were sacrificed right away following establishment of corneal epithelial abrasion models （0 hour group）. The other 48 rabbits were randomly divided into recombinant human epidermal growth factor （EGF） derivative group （positive control group） , normal saline solution group （ negative control group） ,0.25% or 0.5% recombinant human BIGH3 protein eye drops group. Corneal abrasion models were created with alcohol corrosion method with a defect area of 7 mm2. The corresponding eye drops were used separately in 4 groups for four times per day after operation. Experimental eyes were examined by the slit lamp microscope, and fluoreseein vital staining were performed 12,24,36,48,72 hours after operation. Planimetry was performed and the corneal photographs were analyzed with computer software. The rabbits were sacrificed 12,24,36,48 and 72 hours after operation, respectively, and the histopathologieal examination of corneal tissue was carried out. Results No obvious irritation response was seen after administered of eye drops in the recombinant human EGF derivative group, normal saline solution group,O. 25% and O. 5% recombinant human BIGH3 protein eye drops groups. Histopathological examination revealed a full-thickness defect of corneal epithelium after modeling. The defect area was gradually smaller with time lapse, and corneal epithelium migrated from periphery toward the center zone. Corneal epithelial cells increased with time lapse. Compared with normal saline solution group, the de
出处
《中华实验眼科杂志》
CAS
CSCD
北大核心
2013年第11期1006-1010,共5页
Chinese Journal Of Experimental Ophthalmology
基金
基金项目：国家自然科学基金项目（81170832）
黑龙江省自然科学基金项目（QC2010113）
