摘要
目的了解粤东地区2011年婴幼儿手足口病的病原体分型情况及其与临床的关系。方法采集2011年1月至12月汕头大学医学院第二附属医院儿科手足口病住院患儿的咽拭子标本,RT-PCR检测肠道病毒71型(EV71),柯萨奇病毒A组16型(CoxA16),肠道病毒属,并对扩增产物测序鉴定。结果 301例患儿中有214例咽拭子中检测到肠道病毒,病毒阳性率为71.1%,其中EV71 60例,占28.0%,CoxA16 108例,占50.5%,除EV71、CoxA16外的其他型别肠道病毒为46例,占21.5%;91例患儿有明显神经系统损害,其中33例EV71检测阳性(33/60,55.0%),35例CoxA16检测阳性(35/108,32.4%),8例除EV71、CoxA16外其他型别肠道病毒检测阳性(8/46,17.4%),15例肠道病毒检测阴性。除1例由EV71引起的的危重型手足口病死亡外其余300例均痊愈出院。结论粤东地区2011年手足口病病原体主要为CoxA16、EV71,但CoxA16已经成为当年优势病原体,其他型肠道病毒也占有一定比率。而EV71最容易引起神经系统损害和导致重症及危重症,EV71、CoxA16及其他型肠道病毒均可引起神经系统损害。
Objective To investigate the etiology detection and clinical analysis of hand, foot and mouth disease（HFMD）among children of eastern Guangdong in 2011. Methods Throat swabs were collected from the inpatients with HFMD in The Second Affiliated Hospital of Medical College of Shantou University from January to December 2011. Enterovirus genus, Coxsackie virus group A type 16 （CoxA16） and enteroviros type 71 （ EV71 ） were detected by reverse transcription polymerase chain reaction （ RT - PCR） , and finally identified by sequencing. Results 301 throat swabs were collected from inpatients. Enteroviruses were detected in 214 （71.1%）, including 60 （28.0%） of EVTI, 108 （50.5%） of CoxA16 and 46 （21.5%） of other pathogens. Of the 91 patients with nervous system damage, 33 were infected with EV71 （ 33/60, 55.0% ） , 35 were infected with CoxA16 （35/I08, 32.4% , and 8 were infected with other pathogens （8/46, 17.4% ）. One fatal case caused by EV71, the rest of the patients were recovered. Conclusion CoxA16 and EV71 are predominant causative agents of HFMD in eastern Guangdong in 2011, while some patients are caused by other pathogens. However, CoxA16 becomes the first causative agents of HFMD instead of EV71. EVT1 - infec ted HFMD patients are more likely to suffer nervous system damage, severe or critically ill. EV71, CoxA16 and other pathogens can cause HFMD with nervous system damage.
出处
《临床和实验医学杂志》
2013年第19期1541-1544,共4页
Journal of Clinical and Experimental Medicine
基金
2011广东省自然科学基金项目
项目号:2011010005203
