摘要
目的分析子痫前期(PE)孕妇血清和胎盘组织视黄醇结合蛋白4(RBP4)的表达及其与胰岛素抵抗(IR)之间的关系,以探讨RBP4在PE发病中的可能作用机制。方法选择2011年12月至2012年12月于解放军第202医院住院分娩的汉族、单胎孕妇92例为研究对象,按照不同疾病类型,将其分为3组,妊娠期高血压疾病患者28例纳入A组,轻度PE患者30例纳入B组,重度PE患者34例纳入C组,选择同期于本院住院分娩的无妊娠期并发症,且经剖宫产分娩的单胎孕妇32例纳入对照组(D组)。采用酶联免疫吸附法(ELISA)检测其血清RBP4水平,并计算稳态模型的胰岛素抵抗(HOMA-IR)指数;并采用蛋白质印记技术(Western blot)检测各组孕妇胎盘组织RBP4蛋白表达情况(本研究遵循的程序符合解放军第202医院人体试验委员会制定的伦理学标准,得到该委员会批准,分组征得受试对象的知情同意,并与之签署临床研究知情同意书)。结果①C组孕妇空腹血糖(FPG),HOMA-IR指数及血清RBP4水平显著高于另3组,组间比较,差异有统计学意义(P<0.05),B,C组血清空腹胰岛素(FINS)水平高于A,D组,组间比较,差异有统计学意义(P<0.05)。②C组孕妇胎盘组织中RBP4蛋白含量最高,B组胎盘组织中RBP4蛋白含量高于D组,组间比较,差异有统计学意义(P<0.05)。③血清RBP4水平与FPG,HOMA-IR指数以及胎盘组织RBP4蛋白含量呈正相关关系(r=0.277,0.315,0.379;P=0.025,0.010,0.002)。结论 RBP4有可能通过增加IR导致PE的发生。
Objective To analyze the expression of retinol binding protein （RBP）4 in serum and placenta tissue and the relationship among serum RBP4 levels and insulin resistance （IR） in pregnant women with preeclampsia （PE） in order to discuss the role of RBP4 in the pathogenesis of PE. Methods Serum RBP4 levels were measured by enzyme-linked immunosorbent assay （ELISA） in 28 cases of gestational hypertension （group A）, 30 cases of mild PE （group B） ,and 34 cases of severe PE （group C） and 32 cases of normal pregnant women （group D） from December 2011 to December 2012. IR was estimated using the homeostatic model assessment-insulin resistance （HOMA-IR） index. The expression of RBP4 in placenta were detected by Western blot. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of No. 202 Peoplets Liberation Army Hospital. Informed consent was obtained from all participates. Results （1） FPG, HOMA-IR index and serum RBP4 levels in group C were higher than those of other three groups, with significant differences （P〈0.05）. Fasting insulin （FINS） levels in group B, C were higher than that in group A, D, with significant differences （P〈 0.05）. （2） Expression level of RBP4 in the placenta tissue of group C was the highest and expression level of RBP4 in the placenta tissue of group B was higher than that in group D, with significant differences （P〈0.05）. （3） Positive correlation were shown between serum RBP4 levels and FPG, HOMA-IR index, expression levels of RBP4 protein in the placenta （r= 0.277, 0.315, 0.379~ P= 0.025, 0.010, 0.002）. Conclusions Increased RBP4 level may be involved in the pathogenesis of preeclampsia by enhanced IR. [Key words]
出处
《中华妇幼临床医学杂志（电子版）》
CAS
2013年第5期516-519,共4页
Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)
基金
辽宁省博士启动基金(20111121)~~
