摘要
背景:在质谱分析中,任何一种同位素标记相对和绝对定量试剂标记的不同样本中的同一蛋白质表现为相同的质荷比,而在串联质谱中,信号离子表现为不同质荷比(114-121)的峰,因此可以分析得到相关蛋白质的定量信息。目的:建立大鼠急性脊髓损伤后脊髓组织差异蛋白质谱,应用同位素标记相对和绝对定量技术联合LC-MS/MS质谱技术从分子水平来探索脊髓差异蛋白的表达情况。方法:取8只SD大鼠,参照Allen’s等方法建立大鼠急性脊髓损伤模型,随机分为脊髓损伤后0 h组和8 h组,每组各4只。损伤后取脊髓组织,用同位素标记相对和绝对定量技术分析大鼠急性脊髓损伤后脊髓组织的差异蛋白质。结果与结论:共鉴定到了220个差异表达的蛋白,上调的差异蛋白数是116个,下调的差异蛋白有104个。其中相关神经再生的差异蛋白12个,其中上调的有7个,下调的有5个。提示实验中检测到的多种差异蛋白及表达明显的神经生长因子可能作为急性脊髓损伤的生物标记物或可能作为临床管理监测急性脊髓损伤的损伤进程、靶向治疗及评估疗效的有力证据。
BACKGROUND： In mass spectrometry analysis, the same protein in different samples labeled with isobaric tags for relative and absolute quantitation presents the same mass-to-charge ratio, while in the tandem mass spectrometry analysis, the ion signal shows different mass-to-charge ratio （114-121）. Thus the quantitative information of the related proteins can be obtained. OBJECTIVE： To establish the protein spectrum of spinal cord tissue differences proteins after acute spinal cord injury, and to explore the spinal differential protein expression on the molecular level using isobaric tags for relative and absolute quantitation combined with LC-MS/MS mass spectrometry technique. METHODS： Eight Sprague Dawley rats were selected to establish the acute spinal cord injury models using Allen＇s method. The rats were randomly divided into 0 hour spinal cord injury group and 8 hours spinal cord injurygroup, four rats in each group. The spinal cord tissues were collected after injury, and the spinal cord tissue differences proteins were analyzed with isobaric tags for relative and absolute quantitation technique after acute spinal cord injury. RESULTS AND CONCLUSION： Totally 220 differentially expressed proteins were identified in this research, the number of up-regulation proteins was 116 and the number of down-regulation proteins was 104. There were 12 differential proteins related to neural regeneration, and among the 12 proteins, there were seven up-regulation proteins and five down-regulation proteins. The various identified differential proteins and significantly expressed nerve growth factors in this experiment can be used as the biomarkers of acute spinal cord injuryor used as the strong evidence for the clinical management and monitoring of the injury process and target therapy of acute spinal cord injury, as well as the effect evaluation.
出处
《中国组织工程研究》
CAS
CSCD
2013年第41期7248-7254,共7页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(81160218/H0605)"不同时间点鞘内注射甲基强的松龙治疗大鼠急性脊髓损伤的蛋白组学实验研究"~~
