摘要
目的:探讨建立高血压合并糖尿病(Diabetes Mellitus,DM)大鼠模型的方法,并观察模型的稳定性。方法:采用链脲佐菌素(Streptozotocin,STZ)腹腔注射的方法造模。8周龄的SHR大鼠(spontaneously hypertensive rats)16只,随机等分成对照组和造模组。另选8只8周龄WKY大鼠作为正常血压对照组。给予造模组SHR按55 mg/kg体重的剂量腹腔注射STZ,诱导建立糖尿病高血压大鼠动物模型。结果:小剂量STZ(55mg/kg)腹腔注射SHR制备的糖尿病高血压大鼠模型,造模成本低,成模率高,模型稳定。结论:造模组能成功诱导建立糖尿病高血压大鼠模型。
Objective： To investigate the method of establishment the rat model of hypertension and diabetes, and to observe the stability of the model. Methods： Diabetic hypertensive rat model was established by intraperitoneal injection of streptozotocin （STZ）. 16 spontaneously hypertensive rats （SHR） of 8-week-old were randomly divided into the control group and the model group. Replacement of 8 WKY rats of 8-week-old was chosen as normotensive control group. SHR intraperitoneal injection of STZ dose 55mg/kg body weight was given to establish the animal model of diabetic hypertensive rats. Results： Given the SHR by intraperitoneal injection of a small dose of STZ （55mg/kg） can successfully established diabetic hypertensive rat model. This method has the advantages of modeling a high success rate, low cost, built the stable model modeling. Conclusions： The stable and reliable diabetic hypertensive rat model was establish.
出处
《现代生物医学进展》
CAS
2013年第26期5022-5024,共3页
Progress in Modern Biomedicine
基金
国家自然科学基金面上项目(81070209)
