摘要
目的:观察菊米提取液对人宫颈癌HeLa细胞生长的抑制作用。方法:建立裸鼠人宫颈癌HeLa细胞移植模型,将裸鼠随机分为对照组和不同浓度药物组(0.001、0.002、0.005、0.01 mg/ml),观察比较各组荷瘤小鼠宫颈癌细胞的生长情况。并将不同浓度菊米提取液与体外培养的HeLa细胞共同孵育48 h后,采用MTT法、流式细胞术及比色法分别检测菊米提取液对HeLa细胞增殖、凋亡、caspase-3/7酶和caspase-6酶活性的影响。结果:随着药物浓度的增加,对荷瘤小鼠的瘤体抑制率逐步增加。MTT法提示菊米提取液对体外培养的HeLa细胞生长有明显的抑制作用,IC50为0.004 mg/ml,且呈现一定的剂量依赖性;流式细胞术显示随着药物浓度增加促使细胞凋亡发生的作用也增强;与对照组比较,除最低药物浓度组(0.001 mg/ml)以外,其余各药物组对caspase-3/7酶和caspase-6酶活性均有显著增强。结论:菊米提取液在体外可浓度依赖性抑制人宫颈癌HeLa细胞增殖,并可通过增强caspase-3、6、7活性促进其凋亡。
Objective： To explore the inhibition of Jumi（traditional Chinese medicine）extraction on the growth of human cervical cancer cell line HeLa.Methods： Nude mouse model of human cervical cancer HeLa cell transplantation was established.The nude mice bearing cancer were randomly divided into control group and Jumi treated groups with different concentration（0.001,0.002,0.005,0.01 mg/ml）.The growth of cervical cancer cell in experimental mice were measured.Cultured HeLa cells were incubated in culture media with or without Jumi extract for 48 hours.Cell proliferation rate,cell apoptosis,caspase-3/7 and caspase-6 activity were determined by MTT colorimetric assay,flow cytometry analysis and spectrophotometric detection,respectively.Results： With the increase of the concentration of Jumi extract,tumor-bearing mice tumor inhibition rate gradually increased.The proliferation of cultured HeLa cells were significantly inhibited by Jumi extract in a dose-dependent manner.IC50 was 0.004 mg/ml.Apoptosis rates in the cells treated with Jumi extract were higher than those of the control group.Compared with the control group,except for lower Jumi treated group（0.001 mg/ml）,caspase-3/7 and caspase-6 activity were significantly increased in the all Jumi treated groups.Conclusion： Jumi extract can inhibit the proliferation of human cervical cancer cell line HeLa in vitro in a dose-dependent manner and promote cell apoptosis through caspase-3,caspase-7 and caspase-6 pathway.
出处
《中国应用生理学杂志》
CAS
CSCD
2013年第3期275-279,共5页
Chinese Journal of Applied Physiology
基金
浙江省医药卫生一般研究计划(2012KYB057)
关键词
菊米提取液
宫颈癌
增殖
凋亡
Jumi extraction
cervical cancer
proliferation
apoptosis
