摘要
目的研究川芎嗪对大鼠肝纤维化TGF-β1/Smads信号通路的影响。方法 Wistar雄性大鼠50只,随机分为5组:空白组、模型组、川芎嗪组(200 mg/kg组、400 mg/kg组、800 mg/kg组)。用CCl4诱发大鼠肝纤维化,川芎嗪不同剂量组作用大鼠8周后处死并分离肝星状细胞。应用免疫细胞化学法及Western印迹法检测肝星状细胞中TGF-β1、Smad 2/3的表达及分布。结果 TGF-β1、Smad 2/3均主要表达于肝星状细胞中。免疫细胞化学结果示TGF-β1主要在胞核表达,Smad 2/3蛋白则在胞核及胞浆均有表达。CCl4注射诱导后,大鼠肝组织中TGF-β1、Smad 2/3蛋白的表达较空白对照组明显增强(P<0.05)。随着川芎嗪浓度(200、400、800 mg/kg)的升高,TGF-β1、Smad 2/3蛋白在肝组织的表达强度呈逐级递减的趋势,与空白对照组比较,差异有统计学意义(P<0.05)。结论川芎嗪可能通过抑制TGF-β1/Smads信号转导通路,从而发挥其抗肝纤维化的作用。
Objective To study the effects of Tetramethylpyrazine （TMP） on expression of TGF-β1 and Smad 2/3 in hepatic fibrosis in rats. Methods 50 adult male Wistar rats were randomly divided into five groups： normal group, model group, TMP groups （200 mg/kg group, 400 mg/kg group, 800 mg/kg group）. Liver fibrosis models of rats were made by subcutaneously injecting with CC14 HSC were collected in rats after treated by different dosage of tetrameth- ylpyrazin groups at the end of eighth weeks. Immunocytochemistry and Western blotting were used to observe the location of TGF-β1 and Smad 2/3, to test the effect of TMP on the expression of TGF-β1 and Smad 2/3 in HSC. Results The expression of both TGF-β1 and Smad 2/3 protein in rats after injection with CC14 were found chiefly in hepatic stellate cells （HSC）. The test by immunohistochemical method indicated that the expression of TGF-β1 located in the nucleus of HSC, while Smad 2/3 were expressed both in the nucleus and endochylema of HSC and all significantly higher than those in normal rats （P 〈 0.05）. Compared with the control group, the levels of TGF-β1 and Smad 2/3 were decreased significantly in hepatic tissue when the TMP density was increased （P 〈 O. 05）. Conclusion Inhibiting of TGF-β1 Smads signal transduction pathway might be involved in the anti-fibrosis mechanism of TMP.
出处
《胃肠病学和肝病学杂志》
CAS
2013年第10期970-973,共4页
Chinese Journal of Gastroenterology and Hepatology
基金
重庆市科委自然科学基金项目(2006B135428)
关键词
川芎嗪
肝纤维化
转化生长因子B1
SMAD
2
3
肝星状细胞
大鼠
Tetramethylpyrazine
Liver fibrosis
Transforming growth factor 131
Smad 2/3
Hepatic stellatecells
Rats
