摘要
目的研究乙型病毒性肝炎(HBV)患者外周血中乙型病毒性肝炎脱氧核糖核酸(HBV-DNA)载量与白细胞介素12(IL-12)、白细胞介素18(IL-18)及白细胞介素10(IL-10)的相关性。方法随机选取450例HBV-DNA均阳性的HBV患者,于治疗前后各抽取1份血清,采用实时荧光定量PCR法检测外周血中HBV-DNA,用化学发光免疫分析法(CLIA)检测外周血中IL-12、IL-18及IL-10的浓度,并与116名正常对照者进行比较,利用SPSS16软件进行相关性分析。结果 HBV患者组治疗前外周血中IL-12、IL-18及IL-10的浓度均明显高于正常对照组,差异有统计学意义(P<0.05);HBV患者的HBV-DNA载量与IL-12、IL-18、IL-10含量相关性系数分别是r=-0.732、r=-0.856、r=0.645,差异有统计学意义(P<0.05)。HBV患者组治疗后外周血中IL-12、IL-18及IL-10的浓度与正常对照组比较差异均有统计学意义(P>0.05);HBV患者组的HBV-DNA载量与IL-12、IL-18、IL-10含量相关系数分别是r=-0.212、r=-0.335、r=0.234,差异无统计学意义(P>0.05)。结论 HBV-DNA阳性HBV患者血清中HBV-DNA载量与IL-12、IL-18及IL-10含量均具有良好的相关性,其中与IL-18的相关性更佳。
Objective To study the relationship among hepatitis B virus DNA （HBV-DNA）,interleukin 12 （IL-12）,interleukin 18 （IL-18） and interleukin 10 （IL-10） levels in chronic hepatitis B patients.Methods Randomly selected 450 cases of HBV-DNA were positive in a group of patients with HBV before and after treatment the serum,HBV-DNA was detected by realtime-PCR,IL-12,IL-18 and IL-10 were detected by chemiluminescence immunoassay （CLIA） and auto biochemistry analyzer respectively.And 116 cases of normal control group for comparison.The data was analyzed by SPSS 16 for Windows software.Results The IL-12,IL-18 and IL-10 levels of group of patients with HBV were higher than that of normal control group （P＆lt;0.01）.The group of patients with HBV of HBV-DNA copys IL-12 content relevance ：r=0.732,P＆lt;0.01.Comparative IL-18 content relevance ：r =-0.856,P ＆lt;0.01.Comparative IL-10 content relevance ：r =0.645,P ＆lt;0.01.After treatment in patients with HBV in peripheral blood serum concentrations of IL-12,IL-18,IL-10 with the normal control group,no significant difference （P＆gt;0.05）.The connection between in patients with HBV group of HBV-DNA load and IL-12：r=-0.212,P＆gt;0.05;and IL-18：r=-0.335,P＆gt;0.05;and IL-10：r=0.234,P＆gt;0.05.Conclusion Serum HBVDNA capacity related peripheral blood IL-12,IL-18 and IL-10 with HBV-DNA positive that was a good correlation.Among the correlation with IL-18 was better.
出处
《热带医学杂志》
CAS
2013年第8期970-972,共3页
Journal of Tropical Medicine
