摘要
目的探讨细菌性食物中毒的检测过程,为基层卫生部门细菌性食物中毒检测提供一定参考。方法①实时荧光PCR细菌鉴定。②选择性平板直接分离培养,筛选优势菌落进行细菌鉴定。③选择性增菌后分离培养、细菌鉴定。结果综合三种检测实验结果,18起食物中毒中16起检出致病菌,检出率为88.9%;其中13起食物中毒当天检出致病菌,另1起第三天检出致病菌。结论应用该检测过程大大缩短检出时间,且检出率极高。
ObjectiveRetrospective study of bacterial food poisoning in the detection process, to provide a reference for the health department detection of bacterial food poisoning.Methods① Real time fluorescence PCR bacterial identification. ② Selective plate isolation, screening dominant colony of bacteria identification.③ Selective enrichment isolation, identification of bacteria.ResultsThe three kinds of detection results, 18 cases of food poisoning and 16 pathogens were detected, the detection rate was 88.9%; of which 13 cases of food poisoning the pathogenic bacteria were detected on the first day, another third days detection.ConclusionThis detection process greatly shorten the inspection time, and high detection rate.
出处
《中国实用医药》
2013年第29期30-31,共2页
China Practical Medicine
关键词
食物中毒
检测过程
细菌鉴定
荧光PCR
Food poisoning
Detection process
Bacteria detection
Fluorescent PCR
