摘要
本研究构建了一个草甘膦污染土壤的宏基因组文库,利用草甘膦抗性筛选方法从该文库中筛选得到一个草甘膦抗性克隆,测序结果表明,其上存在一个草甘膦氧化还原酶(glyphosate oxidoreductase,简称GOX)的编码基因,命名为goxA。该基因长为1 296 bp,编码一个由431 aa组成的蛋白质。goxA碱基序列与已报道的gox相差6个碱基,编码产物GOXA与GOX蛋白质氨基酸序列相差2个氨基酸。为了验证GOXA的功能,对其进行了原核表达与纯化,活性电泳结果表明,GOXA具有明显的草甘膦氧化还原酶活性,goxA的发现为草甘膦抗性植物的研发提供具有潜在应用价值的基因资源。
In this study, a metagenomic library of glyphosate-contaminated soil was constructed, and a glyphosate-resistant clone was obtained from the library with glyphosate resistance screening method. A novel glyphosate oxidoreductase （GOX） gene was found from the positive clone, which was designated as goxA. The length of goxA was 1296 bp, and its encoding product composed of 431 aa. There were six bases difference between goxA and gox. In addition the encoded amino acid sequence of GOXA had two amino acids difference with GOX. Then prokaryotic expression and purification were done. The active stain result showed that GOXA had obvious glyphosate oxidoreductase activity. In short, the goxA identified in this study can become potential candidate genes for developing glyphosate-tolerant transgenic plants.
出处
《吉林农业科学》
CSCD
北大核心
2013年第5期58-61,共4页
Journal of Jilin Agricultural Sciences
基金
国家高技术研究发展计划"863"重点项目(2007AA020317)
吉林省科技厅重大专项(20086029)
