摘要
目的：通过检测组胺释放考察19种临床常用中药注射剂致大鼠腹腔原代肥大细胞脱颗粒情况，为评价中药注射剂致类过敏性提供参考。方法：应用荧光法检测组胺，并进行条件优化以适用于中药注射剂所致肥大细胞释放组胺的检测。分离大鼠腹腔肥大细胞，分别将19种中药注射剂、阳性药C48／80和肥大细胞混合孵育1h，采用优化的荧光法检测组胺，再计算组胺释放率并进行统计学分析。结果：荧光法检测组胺最佳缩合反应时间为3min，终止反应后7．5～30min缩合物稳定性好；采用间接检测组胺释放率与直接检测组胺释放率两方法结果间无显著差异。痰热清、清开灵、参麦、丹参、血塞通、参附、双黄连、生脉、香丹、热毒宁、复方当归等11种中药注射剂致肥大细胞释放组胺作用明显。脉络宁、黄芪、艾迪、醒脑静、黄芪多糖、红花、盐酸川芎嗪、参芪等8种注射剂未见明显致肥大细胞释放组胺作用。结论：中药注射剂引发的过敏样反应可能大多为类过敏，检测组胺释放分析原代肥大细胞脱颗粒试验可以为评价中药注射剂的致类过敏性提供参考。
Objective： To investigate the stimulation effect of 19 traditional Chinese medicine injections （TCMIs） on rat peritoneal mast cell degranulation in vitro. Method： The optimal reaction factors were found to detect histamine of release caused by TCMIs. And rat peritoneal mast ceils were isolated and purificated as reference described. The cells were then incubated in Tyrode＇s containing 100 mL ·L^-1 TCMI or 4 mg ·L^-1 C48/80 for 1 h. After washing, degranulation was assessed by measuring histamine in cell lysates as previously described, and percentage of degranulated peritoneal mast cell were calculated in a modified method. Result： The optimal reaction time of histamine and O-phthaldialdehyde （OPT） to form a fluorophore is 3 min. The fluorophore is stability during 7.5-30 rain after the reaction was terminated. Percentages of histamine release in two calculation methods showed no difference. The mast ceils, stimulated by Tan-re-qing, Qing-kai-ling, Shen-mai, Dan-shen, Xue-sai-tong, Shen-fu Shuang-huang-lian, Sheng-mai, Xiang-dan, Re-du-ning, Fu-fang-dang-gui, had higher percentages in histamine release than that in control. But, those stimulated by Mai-luo-ning, Huang-qi, Ai-di, Xing-nao-jing, Huang-qi-duo-tang, Hong-hua, Yan-suan-chuang-xiong-qin, Shen-qi had no difference with the control in degranulation. Conclusion： Most hypersusceptibility caused by TCMIs It suggests that rat peritoneal mast cell degranulation test by detecting histamine anaphylactoid reactions caused by TCMIs. may be anaphylactoid reactions release can be used to evaluate
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第21期271-275,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省211工程3期重点学科建设项目(粤发改社【2009】972号)
广东省中医管理局科研课题(2009225)
广东高校优秀青年创新人才培育项目(LYN09049)
关键词
中药注射剂
肥大细胞
脱颗粒
类过敏反应
traditional Chinese medicine injection
mast cells
degranulation
anaphylactoid reaction
