摘要
目的:建立高效液相色谱法检测砂连和胃胶囊中盐酸小檗碱含量的方法。方法:采用Aglient hypersil C18色谱柱(4.6mm×150 mm,5μm),乙腈-水(40:60)(每1 L中加入十二烷基硫酸钠3.4 g,磷酸二氢钠1.7 g,醋酸调整pH3.0)作为流动相,检测波长345 nm,柱温40℃,流速1ml/min。结果:该法在68.9-689 ng(r=0.998,n=9)范围内有较好的线性关系,平均加样回收率为100.14%,ESD为2.01%(n=9)。结论:高效液相色谱法检测砂连和胃胶囊中盐酸小檗碱操作简单,且灵敏度和稳定性高,可作为砂连和胃胶囊有效成分的定量。
Objective： To establish a method for determining the contents of Berberine Hydrochloride in Shalianhewei capsule by HPLC. Methods： Stationary phase was Aglient hypersil C18 （4. 6 mmx 150 mm, 5μm） , mobile phase was acetonitrile-0.2% acetic acid （30： 70） , determination wave length was 345 nm, column temperature was 40 ℃ , flow rate was 1 ml/min. Results ： The method in the range of 43.51 ng/mg - 689 ng/mg（ r = 0. 998,n = 9）, had a good linearity. The average recovery ratio was 100. 14% and RSD was 2.01% （n = 9）. Conclusion： This method of Berberine Hydrochloride in Shalianhewei capsule by HPLC is simple. To detection material usage small, high efficiency, material is simple, fast, high sensitivity, applicable to the higher sensibility and stability. The method can be used to control the quality of Shalianhewei capsule.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2013年第10期1196-1197,共2页
JOURNAL OF BASIC CHINESE MEDICINE
