摘要
目的:采用离体心脏模型建立附子生物检定方法,优化实验条件。方法:通过比较不同实验条件下附子对心脏的作用,筛选检测指标,优化实验条件,从而建立附子生物检定的检测体系。结果:检测体系条件为:采用Langendorff离体心脏灌注装置,K-H液灌注,采用恒温、恒压、恒量的方法给药,以心率为判定指标。结论:所优化建立的实验体系符合附子生物检定的要求。本法有可能成为现行附子质量控制方法的一种补充。
Objective： Using the isolated heart model to establish Aconite bioassay method,Optimizing the experimental conditions. Methods： Comparing the aconite＇ s role of the heart with the different experimental conditions,Screening test indicators,Optimizing the experimental conditions,So as to establish the aconite bioassay detection system. Results： Inspection system conditions： Using Langendorff isolated heart perfusion device,K-H liquid perfusion,the use of constant temperature and pressure,a constant amount of administration,heart rate decision index. Conclusion： The optimization of the experimental system establishes to comply with the requirements of aconite bioassay. Law may become a complement of the existing the aconite quality control methods.
出处
《四川中医》
2013年第9期43-46,共4页
Journal of Sichuan of Traditional Chinese Medicine
基金
国家重点基础研究发展计划(973计划)
课题编号:2009CB522801
国家自然基金面上项目
课题编号:81073047
关键词
附子
质量评价
生物效检定
Aconite quality evaluation
Biological potency test
