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阻断剂对磁致伸缩生物传感器性能的影响 被引量:1

Effects of Blocking Agents to the Performances of Magnetostrictive Biosensors
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摘要 病菌的非特异性结合是影响生物传感器性能主要因素之一,而阻断剂是降低病菌非特异性结合的主要途径之一。本文以李斯特菌为目标病菌,对磁致伸缩生物传感器进行不同的生物加载处理,包括:无生物加载,抗体加载,阻断剂酪蛋白加载,阻断剂牛血清白蛋白加载。通过上述传感器对李斯特菌的检测实验,研究阻断剂的阻断效率以及其对磁致伸缩生物传感器性能的影响。实验结果表明,上述两种阻断剂均可以显著降低李斯特菌与磁致伸缩生物传感器表面的非特异性结合,提高磁致伸缩生物传感器的稳定性能。 Nonspecific binding of bacteria is one of the major issues that influence the performances of biosensors. Blocking agent is an effective way to prevent nonspecific binding. In this paper,Listeria monocytogenes are used as the target analytes, and the surfaces of magnetostrictive biosensors are immobilized with antibodies only, blocking a- gents Casein only and blocking agent Bovine Serum Albumin (BSA) only, respectively. The magnetostrictive biosen- sors with different surface treatment conditions (including:without any treatment, immobilization of antibody only, immobilization of Casein only, and immobilization of BSA only)were used for detection of Listeria Monocytogenes. The efficiency of the two block agents and the effects to the performances of magnetostrictive biosensors are studied. The data shows that the both blocking agents can significantly reduce nonspecific binding of Listeria monocyto- genes and improve the performances of magnetostrictive biosensors.
出处 《太原科技大学学报》 2013年第4期272-275,共4页 Journal of Taiyuan University of Science and Technology
基金 太原科技大学博士科研启动基金(20132004) 美国农业部科研基金(20053439415674A)
关键词 磁致伸缩生物传感器 李斯特菌 非特异性结合 阻断剂 magnetostrictive biosensor, Listeria monocytogenes, nonspecific binding, blocking agent
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