摘要
目的:探究临床生化检验测量不确定度。方法:针对性地采用相对的方法进行测量,采用间接选择的电极法测量Cl-、K+;采用速率法测量谷氨酰转肽酶(GGT);采用双缩脲的终点法测量总蛋白(TP);采用溴甲酚绿显示终点法测量白蛋白(Alb);采用酶法测量肌酐(Cr)、采用脲酶紫外速率法测量尿素氮(BUN);采用尿酸酶法测量尿酸(UA);按照不确定度(U%)公式U%=2CV%计算结果。结果:Cl-、K+、GGT、TP、Alb、BUN、Cr、UA的不确定度分别是4.18%、3.26%、8.88%、5.18%、5.90%、10.26%、6.12%、8.16%。结论:测量不确定度可以反映出本室实验条件下检测结果的准确度。
Objective： To explore the clinical biochemical measurement uncertainty.Method： The method of relative measurement were used, Cl , K＋ were measured by indirect selective electrode method; glutamyl transpeptidase （ GGT ） was measured by rate method; biuret＇s （ TP ） was measured by the total protein end-point method; albumin end-point method （Alb）was measured by bromocresol green display terminal method; creatinine （ Cr ） was measured by enzyme method, blood urea nitrogen （ BUN ） was measured by urease UV rate method; uric acid （ UA ） was measured by uricase method; according to the uncertainty （ U% ） calculation formula U%=2CV%.Result： The uncertainty of Cl-, K＋, GGT, TP, Alb, BUN, Cr, UA respectively was 4.18%, 3.26%, 8.88%, 5.18%, 5.90%, 10.26%, 6.12%, 8.16%.Conclusion： The uncertainty of measurement can reflect the experimental conditions, the accuracy of detection results.
出处
《中国医学创新》
CAS
2013年第28期90-91,共2页
Medical Innovation of China
关键词
临床生化检验
不确定度
探究
Clinical biochemistry test
Uncertainty
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