摘要
The aim of the study was to find the interrelations between the activity of intracellular dehydrogenases, abundance of microorganisms, and the level of soil DNA in the Mollic Gleysol profile, with notification on the dominant DNA form （extra-or intra-cellular）, depending on the type of land use. Two neighbouring meadows were selected for investigations： one systematically cultivated and fertilized and the other deprived of any effect of anthropogenic activity, used as a control. We have demonstrated that dehydrogenase activity （DHA）, the DNA content and microbial abundance strongly depended on the type of land use. DHA exhibited a significant correlation with the DNA content （r = 0.99^＊＊＊ and r = 0.74^＊, for cultivated and control sites, respectively）. This relationship with such a high r value might suggest domination of the intracellular form of DNA in the cultivated meadow, which is also confirmed by the c.a. 13% increase in microorganism abundance in the cultivated soil. The optimal conditions for microbial activities were defined by the significant positive interrelationships between microbial abundance and the total organic carbon content, and a negative correlation with pH, redox potential and soil bulk density.
