摘要
目的探讨人参皂苷Rg1(ginsenoside Rg1)对脑缺血再灌注大脑皮层细胞死亡方式的影响。方法健康SD大鼠随机分为假手术组(Sham组)、缺血再灌注组(Model组)、人参皂苷Rg1 10、20、40 mg/kg处理组、尼莫地平处理组(阳性对照组),每组15只。各组于术前5 d至取材当日分别注射生理盐水(Sham、Model组)、人参皂苷Rg1(Rg1处理组)、尼莫地平(阳性对照组)。采用右侧大脑中动脉栓塞法制备脑缺血模型,动物清醒后进行神经功能评分和TTC染色验证模型是否成功;采用免疫组化法和免疫印迹法定性定量检测大脑皮层缺血再灌注24h后多聚二磷酸腺苷核糖聚合酶-1(poly ADP-ribose polymerase-1,PARP-1)、半胱氨酸蛋白酶-3(caspase-3)的表达。结果免疫组化和免疫印迹结果显示,Sham组大脑皮层区可见PARP-1、及少量caspase-3阳性细胞;Model组可见大量PARP-1阳性细胞及caspase-3阳性细胞;人参皂苷Rg1处理组神经功能缺损评分及PARP-1、caspase-3的表达显著低于Model组。结论人参皂苷Rg1预处理对大鼠脑缺血再灌注具有保护作用,其机制可能与下调脑组织PARP-1、caspase-3的表达,对抗脑细胞凋亡和胀亡有关。
Objective To study the mechanism ofginsenoside Rgl after cerebral ischemia-reperfusion in adult rats. M~ Rats were randomly divided into the sham-operative group （sham group）, Model group, ginsenoside Rgl 10,20,40 mg/kg groups, Nimodipine group （drug control group）. Cerebral ischemia-reperfusion （CIR） model was made by occluding the middle cerebral artery in rats. After CIR, the neurological deficit score was evaluated and IHC and Western blot technique was used to observe the expressions of PARP-1 and caspase-3. Results Compared with Model group, the neurological deficit scores and the expressions of PARP-1 and caspase-3 were significantly decreased. Concltmion Ginsenoside Rgl can protect the brain cells fi＇om damage after C1R injury by down-regulating the expression of PARP-1 and caspase-3.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2013年第5期555-559,共5页
Chinese Journal of Clinical Anatomy
基金
辽宁省自然科学基金项目(201102139)
