摘要
目的：测定平喘颗粒中盐酸小檗碱的含量，建立平喘颗粒的质量控制方法。方法：色谱柱为Hy—persilODS2 C_18（250mm×4．6mm，5μm）；流动相为乙腈：水（100mL加十二烷基磺酸钠0．4g，磷酸调节pH值为4），体积比为（50：50）；检测波长为345nm。结果：盐酸小檗碱在0．1854～0．927μg范围内线性关系良好，r=0．9999。结论：该法简便、准确、重复性好，可作为平喘颗粒的质量控制方法。
Objective： To establish a method of quality control for pingchuan Granule. Methods： The colunm was C18 （250mm × 4.6mm, 5μm）. The mobile phase was acetonitrile ： water（50： 50）（0.4g SDS in 100mL mobile phase, pH = 4）, the detective wave length was at 265nm with the flow rate of 1.0mL/min. Results：The Berberine hydrochloride was linear within the concentration range of 0. 1854-0. 927μg（r=0. 999 9）. The average recovery was 99.24%0. Conclusion： This method is simple,practical,strong specificity and good reproducibility, it can be used for the quality control of pingchuan Granule.
出处
《亚太传统医药》
2013年第10期29-30,共2页
Asia-Pacific Traditional Medicine
关键词
平喘颗粒
盐酸小檗碱
HPLC
Pingchuan Granule
Berberine Hydrochloride
RP-HPLC
