摘要
越来越多的研究表明肿瘤细胞中存在肿瘤干细胞,它与肿瘤的起始、生长、转移及化疗抗性有密切关系。食管鳞癌细胞中也被发现具有干细胞特性的肿瘤细胞,这类细胞具有自我更新、分化潜能、裸鼠成瘤和化疗抗性,这类细胞将在肿瘤靶向治疗中发挥重要的作用。目前培养和分离食管鳞癌干细胞的方法主要有免疫荧光激活细胞分离法、免疫磁珠激活细胞分离法、悬浮培养法、侧群细胞分离法等。本文对当前食管鳞癌干细胞的研究方法、生物学特性及不足进行了综述,并认为食管鳞癌干细胞需要联合多个细胞标记进行研究。
Increasing evidence suggests that many types of cancers contain a population of cells that display stem cell properties. These cells are called cancer stem cells (CSCs), which are closely related to tumor initiation, growth, metastasis and chemoresistance. CSCs are also found in esophageal squamous cell carcinoma (ESCC). These cells are characterized by potential of self-renewal and differentiation, tumor formation in nude mice and chemotherapy resistance, and thus may play an important role in targeted cancer therapies. Current methods for culturing and sorting CSCs in ESCC mainly include fluorescence activated cell sorting (FACS), magnetic activated cell sorting (MACS), suspension culture, and side population (SP) cell sorting. In this review, we focus on current research methods for CSCs in ESCC, their bio- logical characteristics and areas for improvement. We believe that a combination of multiple cell-surface makers is needed for research of CSCs in ESCC.
出处
《中国胸心血管外科临床杂志》
CAS
2013年第5期583-587,共5页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
关键词
食管鳞状细胞癌
肿瘤干细胞
细胞表面标记
悬浮培养
侧群细胞
Esophageal squamous cell carcinoma
Cancer stem cells
Cell-surface marker
Suspension culture
Side population