摘要
采用碱提的方法从杏鲍菇中提取得到水溶性粗多糖PEAP,并经DEAE-52纤维素和SephadexG-150柱层析法分离纯化,获得一种新的杏鲍菇多糖组分PEAP-1.通过HPLC、GC、IR、刚果红实验、原子力显微镜（AFM）,以及环境电镜扫描（SEM）分析方法,研究了PEAP-1的结构形貌.HPLC和GC结果表明,PEAP-1为均一多糖,相对分子质量为450 kDa,单糖基由葡萄糖和半乳糖组成,其摩尔比为16.9∶0.37.在红外光谱图中,832 cm-1和919 cm-1附近出峰,表明在PEAP-1中存在吡喃糖环结构.刚果红实验和AFM图像显示,PEAP-1的多分支结构中没有三股螺旋构型,单个分子呈无规则链状,直径在0.13～1.2 nm之间.同时,在SEM检测下PEAP-1呈片状、杆状和网状形貌.
In this manuscript,the water-soluble crude polysaccharides PEAP was extracted from Pleurotus Eryngii（PE） by alkali solution and fractionated by DEAE-52 cellulose and Sephadex G- 150,obtaining a novel polysaccharide composition termed PEAP-1. Furthermore,the structural characteristics of PEAP-1 was analyzed by HPLC,GC,IR,Congo red test,AFM and SEM methods. The results of HPLC and GC analysis indicated that PEAP-1 was a homogeneous polysaccharide whose average molecular weight is 450 kDa and monoaccharide is composed of Glu and Gal at a molar ratio of 16.9：0.37. Moreover,the peak near 832 cm-1 and 919 cm-1 suggested the presence of pyranose ring in PEAP-1 from the infrared spectroscopy. The Congo red test and atomic force microscope （AFM） images showed that PEAP-1 has no triple-helix structure but with multi- branched frame,and its single molecular presents random coil chain with a diameter of 0.13-1.2 nm. Meanwhile,the flake,rods and mesh morphology of PEAP-1 was detected using SEM.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2013年第9期951-956,共6页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(10874108)
陕西省自然科学基础研究计划项目(SJ08A16)
关键词
碱提杏鲍菇多糖
分离纯化
结构初探
原子力显微镜
环境电镜扫描
Alkali -extractable polysaccharides from Pleurotus Eryngii,purification,Preliminary Structure Analysis, AFM, SEM
