摘要
目的:研究不同盐碱胁迫对党参种子萌发和幼苗生长的影响,探寻提高其抗性能力的途径。方法:测定不同盐碱胁迫下党参种子的发芽势(G v)、发芽率(G r)、发芽指数(G i)和复萌率,幼苗含水量、叶绿素含量、可溶性蛋白质含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化物酶(POD)活性。结果:对党参种子萌发的抑制影响为Na2CO3>NaCl+Na2CO3>NaCl。随着3种盐碱处理的浓度逐渐增大,党参幼苗中叶绿素含量、幼苗含水量、可溶性蛋白质含量逐渐降低,且与对照组间均有显著性差异(P<0.05),均与盐碱处理浓度呈负相关性。但丙二醛(MDA)含量逐渐增大,与盐碱处理浓度呈正相关性。SOD、POD的活性均随盐碱处理浓度的增大而出现"先上升,后下降"的趋势。结论:不同盐碱胁迫对党参种子的萌发及幼苗的生长都有抑制作用,但抑制程度不同,其中Na2CO3>NaCl+Na2CO3>NaCl。
Objective ：To improve salt resistance of Codonopsis pilosula seeds and seedlings under different salt-alkaline stress, the seed germination and physiological characteristics of seedlings were studied. Methods：Several physiological indexes of Codonopsis pilosula under different salt-alkaline stress were measured, such as the germination vigor, germination rate, relative germination rate and recovery germination rate, as well as others indexes like the contents of chlorophyll, soluble protein,water and MDA and the activities of POD and SOD. Results ： The inhibitory effects of three salts on seed germination were Na2 CO3 〉 NaC1 ＋ Na2 CO3 〉 NaC1 with an increased salt concentration. The contents of chlorophyll, soluble protein and water were found significant different （ P 〈 0. 05 ） from the negative control group, along with the decreased of these indexes. However, positive correlation existed between MDA content and salt treatment concentration. In addition, the change trend of SOD and POD activity were similar. They were increased firstly, and then decreased as the stress intensity extended. Conclusion：The seeds and seedlings under different salt-alkaline stress were inhibited, with different inhibitory extent as Na2CO3 〉 NaC1 ＋ Na2CO3 〉 NaC1.
出处
《中药材》
CAS
CSCD
北大核心
2013年第7期1039-1043,共5页
Journal of Chinese Medicinal Materials
基金
国家科技支撑计划项目(2007BAI37B01)
国家中医药管理局2012年中医药行业科研专项(201207002)
河西学院青年教师科研基金(QN2012-13)
关键词
党参
盐碱胁迫
种子萌发
生理特性
Codonopsis pilostda （ Franch. ） Nannf.
Salt-alkaline stress
Seed germination
Physiological characteristics
