摘要
目的:探讨糖尿病大鼠肾脏中4羟基壬烯醛(4-HNE)的表达情况及其作用机制。方法:链脲佐菌素(STZ)60 mg/kg一次性腹腔注射构建糖尿病大鼠模型组(D组),并设空白对照组(C组),分别检测大鼠4周、8周及12周的血肌酐、尿素氮、三酰甘油、胆固醇及24 h尿蛋白定量,光镜下观察肾脏病理改变,免疫组化及Western-blot观察各组大鼠肾脏4-HNE的表达情况。结果:D组大鼠4周、8周及12周的肌酐、尿素氮、三酰甘油及胆固醇逐渐升高,并均高于同一时间点的C组,结果差异有统计学意义(P<0.05),随病程的延长D组大鼠肾脏病理改变逐渐加重,基底膜逐渐增厚,细胞外基质逐渐增多,C组病理改变不明显。免疫组化及western-blot显示D组大鼠4周、8周及12周肾组织中4-HNE的表达逐渐升高,并明显高于C组,差异有统计学意义(P<0.05)。结论:糖尿病大鼠肾脏中4-HNE随着病程延长其表达量逐渐升高,提示脂质过氧化参与了糖尿病肾病的发生及进展。
Objective： To investigate the expression of 4 - hydroxynonenal （4 - HNE） in the kidney of diabetic rats induced by STZ and its mechanism. Methods：25 rats were given STZ （60 mg/kg） by intraperitoneal injection to establish diabetic rat models. The rats whose blood glucose were higher than 16.7 mmol/L 72 hours later and last for one week could be considered as the successful diabetic rat models. 24 diabetic rats were putted into diabetic group （group D）, and 24 healthy rats were considered as control group （group C）. The serum creatinine （Scr）, blood urea nitrogen （BUN）, triglyceride （TG）, total cholesterol （TC） and 24 -hours urinary protein were measured at the 4th, 8th and 12th weeks. PAS staining and HE staining were used to evaluate the pathological changes of the kidney. The immunohistochemistry and Western - blot were used to detect the expression of 4 - HNE in renal tissue. Results：Scr, BUN, TG, TC and 24 - hours urinary protein in group D increased at the end of 4th, 8th and 12th weeks, and were much higher than those in group C at the same time, the differences had statistical significance （P 〈 0.05 ）. The pathological changes of the kidney in group D were not distinct at the end of 4th weeks, but the pathological changes were much more serious than group C at 8 th weeks and 12 th weeks. The expression of 4 -HNE in group D were much higher than group C at 4th,8th and 12 th weeks （P 〈 0.05 ）. Conclusion：The experssion of 4 -HNE increases in diabetic rat model, which indicates that lipid peroxidation may take part in the progression of diabetic nephropathy.
出处
《中国中西医结合肾病杂志》
2013年第9期773-775,I0002,共4页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
青岛市科技局基金资助项目[No.2012-1-3-4-(2)-nsh]
