摘要
根据得到的atp1基因序列设计CDS区特异引物,分别以红麻不育系和保持系的DNA和RNA反转录得到的cDNA为模板扩增atp1基因,测序并进行序列比对后发现,以DNA为模板和以cDNA为模板扩增得到的基因序列在不育系和保持系间序列完全一致,这暗示不育性状并非碱基变异造成。同时,使用半定量RT-PCR技术较全面地分析了atp1基因在红麻中的转录表达特征,结果显示:atp1基因在不育系和保持系的根、茎、叶以及花瓣、花药、雌蕊中都表达,但在不育系的叶片和花药中表达下降,暗示造成不育的原因可能与atp1基因的表达量下降有关。研究为下一步构建表达载体进行转基因表达研究提供了基础,为揭示雄性不育相关基因导致雄性不育的机理提供一定依据。
Specific primers of CDS were designed according to CDS region predicted.The atp1 genes were amplified using DNA and cDNA as template in kenaf male sterile line and its maintainer line respec-tively.The sequences of atp1 gene amplified from DNA and cDNA had no difference between kenaf male sterile line and its maintainer line by sequencing and sequence alignment.It suggested that the sterility was not caused by base mutation of atp1 gene.Semi-RT-PCR analysis indicated that atp1 gene ex-pressed in root, stem, leaf, petal, anther and pistil, but the expression level in leaf and anther of male sterile line was lower than maintainer line , which suggested that the male sterility in kenaf was possibly related to the expression decrease of atp1 gene.
出处
《中国麻业科学》
2013年第5期221-225,共5页
Plant Fiber Sciences in China
关键词
红麻
质核互作雄性不育
功能分析
kenaf
cytoplasmic-nuclear male sterility
gene analysis
